A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

被引：6

作者：

Svendsen, Claus Bo ^{[1
]}

Boye, Mette ^{[2
]}

Struve, Carsten ^{[1
]}

Krogfelt, Karen A. ^{[1
]}

机构：

[1] Statens Serum Inst, Dept Bacteriol Mycol & Parasitol, DK-2300 Copenhagen S, Denmark

[2] Tech Univ Denmark, Natl Vet Inst, Copenhagen, Denmark

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 76卷 / 03期

关键词：

In situ hybridization; Fluorescence; Rickettsia; Archival samples; TARGETED OLIGONUCLEOTIDE PROBES; SPOTTED-FEVER; IDENTIFICATION; POPULATIONS; DIAGNOSIS; TISSUE;

D O I：

10.1016/j.mimet.2008.10.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Rickettsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：301 / 304

页数：4

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