An Emerging Clone, Klebsiella pneumoniae Carbapenemase 2-Producing K. pneumoniae Sequence Type 16, Associated With High Mortality Rates in a CC258-Endemic Setting

被引:53
作者
Andrey, Diego O. [1 ,2 ,3 ,8 ,9 ]
Dantas, Priscila Pereira [4 ]
Martins, Willames B. S. [1 ,5 ]
De Carvalho, Fabiola Marques [6 ]
Almeida, Luiz Gonzaga Paula [6 ]
Sands, Kirsty [1 ]
Portal, Edward [1 ]
Sauser, Julien [3 ,7 ]
Cayo, Rodrigo [5 ]
Nicolas, Marisa F. [6 ]
Vasconcelos, Ana Tereza R. [6 ]
Medeiros, Eduardo A. [4 ]
Walsh, Timothy R. [1 ]
Gales, Ana C. [5 ]
机构
[1] Cardiff Univ, Dept Med Microbiol, Div Infect & Immun, Cardiff, Wales
[2] Geneva Univ Hosp, Serv Infect Dis, Geneva, Switzerland
[3] Fac Med, Geneva, Switzerland
[4] Univ Fed Sao Paulo, Hosp Epidemiol Comm, Dept Internal Med, Hosp Sao Paulo,Div Infect Dis,Escola Paulista Med, Sao Paulo, Brazil
[5] Univ Fed Sao Paulo, Escola Paulista Med, Lab Alerta, Dept Med,Discipline Infectol, Sao Paulo, Brazil
[6] Natl Lab Sci Comp, Petropolis, RJ, Brazil
[7] Geneva Univ Hosp, Infect Control Program, Geneva, Switzerland
[8] Geneva Univ Hosp, Serv Malad Infect, Geneva, Switzerland
[9] Med Sch, Geneva, Switzerland
基金
瑞士国家科学基金会; 英国医学研究理事会; 英国生物技术与生命科学研究理事会;
关键词
carbapenem-resistant Enterobacteriaceae; KPC; bloodstream infections; CC258; Klebsiella pneumoniae; MOLECULAR EPIDEMIOLOGY; ENTEROBACTERIACEAE; SPREAD; DISSEMINATION; OUTBREAK; BRAZIL; ST437; ST11;
D O I
10.1093/cid/ciz1095
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Carbapenemase-producing Klebsiella pneumoniae has become a global priority, not least in low- and middle-income countries. IIere, we report the emergence and clinical impact of a novel Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-KP) sequence type (ST) 16 clone in a clonal complex (CC) 258-endemic setting. Methods. In a teaching Brazilian hospital, a retrospective cohort of adult KPC-KP bloodstream infection (BSI) cases (January 2014 to December 2016) was established to study the molecular epidemiology and its impact on outcome (30-day all-cause mortality). KPC-KP isolates underwent multilocus sequence typing. Survival analysis between ST/CC groups and risk factors for fatal outcome (logistic regression) were evaluated. Representative isolates underwent whole-genome sequencing and had their virulence tested in a Galleria larvae model. Results. One hundred sixty-five unique KPC-KP BSI cases were identified. CC258 was predominant (66%), followed by ST16 (12%). The overall 30-day mortality rate was 60%; in contrast, 95% of ST16 cases were fatal. Patients' severity scores were high and baseline clinical variables were not statistically different across STs. In multivariate analysis, ST16 (odds ratio [OR], 21.4; 95% confidence interval [CI], 2.3-202.8; P = .008) and septic shock (OR, 11.9; 95% CI, 4.2-34.1; P < .001) were independent risk factors for fatal outcome. The ST16 clone carried up to 14 resistance genes, including bla(KPC-)(2) in an IncFIBpQIL plasmid, KL51 capsule, and yersiniabactin virulence determinants. The ST16 clone was highly pathogenic in the larvae model. Conclusion. Mortality rates were high in this KPC-KP BSI cohort, where CC258 is endemic. An emerging ST16 clone was associated with high mortality. Our results suggest that even in endemic settings, highly virulent clones can rapidly emerge demanding constant monitoring.
引用
收藏
页码:E141 / E150
页数:10
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