Impedance studies of bio-behavior and chemosensitivity of cancer cells by micro-electrode arrays

被引:95
作者
Liu, Qingjun [1 ,2 ,3 ]
Yu, JinJiang [1 ]
Xiao, Lidan [1 ,2 ]
On Tang, Johnny Cheuk [4 ]
Zhang, Yu [1 ]
Wang, Ping [2 ,3 ]
Yang, Mo [1 ]
机构
[1] Hong Kong Polytech Univ, Dept Hlth Technol & Informat, Kowloon, Hong Kong, Peoples R China
[2] Zhejiang Univ, Dept Biomed Engn, Biosensor Natl Special Lab, Key Lab Biomed Engn,Educ Minist, Hangzhou 310027, Zhejiang, Peoples R China
[3] Chinese Acad Sci, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China
[4] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
基金
中国国家自然科学基金;
关键词
Cell-based biosensor; Micro-electrode arrays; Cancer; Anti-cancer drug; Impedance spectroscopy; Chemosensitivity; MONITOR CHANGES; ASSAY; CYTOTOXICITY; BIOSENSORS; ADHESION; GROWTH; SHAPE;
D O I
10.1016/j.bios.2008.07.044
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Impedimetric analysis on adherently growing cells by micro-electrodes provides information related to cell number, cell adhesion and cellular morphology. In this study, cell-based biosensor with micro-electrode arrays (MEAs) was used to monitor the culture behavior of mammalian cancer cells and evaluate the chemosensitivity of anti-cancer drugs using electrochemical impedance spectroscopy. The platinum electrode arrays were fabricated by semiconductor technology to a 10 x 10 pattern, with diameter of 80 mu m of each electrode. The human oesophageal cancer cell lines (KYSE 30) were cultured on the Surface of the electrodes with the pre-coated fibronectin, the connecting protein for tumor cells metastasis and adhesion in extracellular Matrix. Morphology changes during cells adhesion, spreading, and proliferation can be detected by impedimetric analysis in a real time and non-invasive way. Cisplatin was added to cells for potential drug screening applications. The experimental results show that this well-known anti-cancer drug has characteristic chemosensitivity effects on KYSE 30 cells which can be detected by MEA. Thus, this cell-based chip provides a useful analytical method for cancer research. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:1305 / 1310
页数:6
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