共 21 条
Impedance studies of bio-behavior and chemosensitivity of cancer cells by micro-electrode arrays
被引:95
作者:
Liu, Qingjun
[1
,2
,3
]
Yu, JinJiang
[1
]
Xiao, Lidan
[1
,2
]
On Tang, Johnny Cheuk
[4
]
Zhang, Yu
[1
]
Wang, Ping
[2
,3
]
Yang, Mo
[1
]
机构:
[1] Hong Kong Polytech Univ, Dept Hlth Technol & Informat, Kowloon, Hong Kong, Peoples R China
[2] Zhejiang Univ, Dept Biomed Engn, Biosensor Natl Special Lab, Key Lab Biomed Engn,Educ Minist, Hangzhou 310027, Zhejiang, Peoples R China
[3] Chinese Acad Sci, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China
[4] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
基金:
中国国家自然科学基金;
关键词:
Cell-based biosensor;
Micro-electrode arrays;
Cancer;
Anti-cancer drug;
Impedance spectroscopy;
Chemosensitivity;
MONITOR CHANGES;
ASSAY;
CYTOTOXICITY;
BIOSENSORS;
ADHESION;
GROWTH;
SHAPE;
D O I:
10.1016/j.bios.2008.07.044
中图分类号:
Q6 [生物物理学];
学科分类号:
071011 ;
摘要:
Impedimetric analysis on adherently growing cells by micro-electrodes provides information related to cell number, cell adhesion and cellular morphology. In this study, cell-based biosensor with micro-electrode arrays (MEAs) was used to monitor the culture behavior of mammalian cancer cells and evaluate the chemosensitivity of anti-cancer drugs using electrochemical impedance spectroscopy. The platinum electrode arrays were fabricated by semiconductor technology to a 10 x 10 pattern, with diameter of 80 mu m of each electrode. The human oesophageal cancer cell lines (KYSE 30) were cultured on the Surface of the electrodes with the pre-coated fibronectin, the connecting protein for tumor cells metastasis and adhesion in extracellular Matrix. Morphology changes during cells adhesion, spreading, and proliferation can be detected by impedimetric analysis in a real time and non-invasive way. Cisplatin was added to cells for potential drug screening applications. The experimental results show that this well-known anti-cancer drug has characteristic chemosensitivity effects on KYSE 30 cells which can be detected by MEA. Thus, this cell-based chip provides a useful analytical method for cancer research. (C) 2008 Elsevier B.V. All rights reserved.
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页码:1305 / 1310
页数:6