Effects of phospholipase A2 inhibitors on Ca2+ oscillations in pancreatic acinar cells

被引：10

作者：

Mizuno, N ^{[1
]}

Naruse, S ^{[1
]}

Kitagawa, M ^{[1
]}

Ishiguro, H ^{[1
]}

Hayakawa, T ^{[1
]}

机构：

[1] Nagoya Univ, Sch Med, Dept Internal Med 2, Showa Ku, Nagoya, Aichi 4668560, Japan

来源：

PANCREAS | 2000年 / 20卷 / 01期

关键词：

pancreatic acini; Ca2+ oscillations; amylase secretion; sPLA(2); cPLA(2); PLA(2) inhibitor;

D O I：

10.1097/00006676-200001000-00011

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

High-affinity cholecystokinin (CCK) receptors were reported to be coupled with phospholipase A(2) (PLA(2))arachidonic acid (AA) pathways to mediate Ca2+ oscillations and amylase secretion in rat pancreatic acinar cells. To investigate which types of PLA(2) were involved in PLA(2)-AA pathways, the effects of specific inhibitors for type II and type IV PLA(2) on Ca2+ oscillations and amylase secretion were studied in isolated rat pancreatic acini. An inhibitor of type TV (cytosolic) PLA(2), AACOCF(3) inhibited Ca2+ oscillations elicited by CCK-8 (30 pM) and JMV-180 (100 nM). AACOCF(3) inhibited amylase secretion stimulated by JMV-180 and low concentrations of CCK-8 (less than or equal to 30 pM). On the other hand, an inhibitor of type II (secretory, nonpancreatic) PLA(2) had no effects on Ca2+ oscillations and amylase secretion stimulated by CCK-8 and JMV-180. These results suggest that high-affinity CCK receptors are coupled to cytosolic PLA(2) to mediate Ca2+ oscillations and amylase secretion in rat pancreatic acinar cells.

引用

页码：77 / 83

页数：7

共 26 条

[1] ACTIVATION OF PHOSPHOLIPASE-A AND PHOSPHOLIPASE-C IN HUMAN-PLATELETS EXPOSED TO EPINEPHRINE - ROLE OF GLYCOPROTEIN-IIB GLYCOPROTEINS-IIIA AND DUAL ROLE OF EPINEPHRINE [J].

BANGA, HS ;

SIMONS, ER ;

BRASS, LF ;

RITTENHOUSE, SE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (23) :9197-9201

[2] Photodynamic triggering of calcium oscillation in the isolated rat pancreatic acini [J].

Cui, ZJ ;

Kanno, T .

JOURNAL OF PHYSIOLOGY-LONDON, 1997, 504 (01) :47-55

[3]

DENNIS EA, 1994, J BIOL CHEM, V269, P13057

[4]

Elliott Austin C., 1997, Digestion, V58, P69

[5]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[6] DUAL EFFECTS OF CHLOROBUTANOL ON SECRETORY RESPONSE AND INTRACELLULAR CA2+ DYNAMICS IN ISOLATED PANCREATIC ACINI OF THE RAT [J].

HABARA, Y ;

KANNO, T .

BRITISH JOURNAL OF PHARMACOLOGY, 1993, 109 (03) :685-692

[7] Potent inhibitors of secretory phospholipase A2: Synthesis and inhibitory activities of indolizine and indene derivatives [J].

Hagishita, S ;

Yamada, M ;

Shirahase, K ;

Okada, T ;

Murakami, Y ;

Ito, Y ;

Matsuura, T ;

Wada, M ;

Kato, T ;

Ueno, M ;

Chikazawa, Y ;

Yamada, K ;

Ono, T ;

Teshirogi, I ;

Ohtani, M .

JOURNAL OF MEDICINAL CHEMISTRY, 1996, 39 (19) :3636-3658

[8] Ca2+ signaling in the pancreatic acinus [J].

Kanno, T .

PANCREAS, 1998, 16 (03) :273-276

[9] Structure, function and regulation of Ca2+-sensitive cytosolic phospholipase A2 (cPLA2) [J].

Kramer, RM ;

Sharp, JD .

FEBS LETTERS, 1997, 410 (01) :49-53

[10] MAMMALIAN NONPANCREATIC PHOSPHOLIPASES-A(2) [J].

KUDO, I ;

MURAKAMI, M ;

HARA, S ;

INOUE, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1170 (03) :217-231

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