Surface Plasmon Resonance for Cell-Based Clinical Diagnosis

被引:115
作者
Yanase, Yuhki [1 ]
Hiragun, Takaaki [1 ]
Ishii, Kaori [1 ]
Kawaguchi, Tomoko [1 ]
Yanase, Tetsuji [1 ]
Kawai, Mikio [1 ]
Sakamoto, Kenji [2 ]
Hide, Michihiro [1 ]
机构
[1] Hiroshima Univ, Grad Sch Biomed Sci, Div Mol Med Sci, Dept Dermatol,Minami Ku, Hiroshima 7348551, Japan
[2] Kyushu Inst Technol, Ctr Microelect Syst, Iizuka, Fukuoka 8208502, Japan
关键词
surface plasmon resonance (SPR); SPR imaging (SPRI); biosensor; clinical diagnosis; diagnosis of allergy; diagnosis of cancer; optic fiber SPR; BIOMOLECULAR INTERACTION ANALYSIS; EPIDERMAL-GROWTH-FACTOR; LABEL-FREE; LIVING CELLS; SENSITIVITY-ENHANCEMENT; BIOSENSOR DETECTS; MAST-CELLS; SPR; SENSORS; ACTIVATION;
D O I
10.3390/s140304948
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Non-invasive real-time observations and the evaluation of living cell conditions and functions are increasingly demanded in life sciences. Surface plasmon resonance (SPR) sensors detect the refractive index (RI) changes on the surface of sensor chips in label-free and on a real-time basis. Using SPR sensors, we and other groups have developed techniques to evaluate living cells' reactions in response to stimuli without any labeling in a real-time manner. The SPR imaging (SPRI) system for living cells may visualize single cell reactions and has the potential to expand application of SPR cell sensing for clinical diagnosis, such as multi-array cell diagnostic systems and detection of malignant cells among normal cells in combination with rapid cell isolation techniques.
引用
收藏
页码:4948 / 4959
页数:12
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