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O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B
被引:1
|作者:
Di Guida, Rossella
[1
]
Casillo, Angela
[1
]
Corsaro, Maria Michela
[1
]
机构:
[1] Univ Naples Federico II, Dept Chem Sci, Complesso Univ Monte S Angelo,Via Cintia 4, I-80126 Naples, Italy
关键词:
Lipopolysaccharide;
NMR;
Psychrotolerant;
Pseudomonas;
Gulopyranuronamide;
PSYCHROPHILIC BACTERIUM;
CAPSULAR POLYSACCHARIDE;
LIPOPOLYSACCHARIDE;
LIPOOLIGOSACCHARIDE;
CHAIN;
SUGARS;
D O I:
10.1016/j.carres.2020.108125
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: -> 4)-alpha-L-GulpNA-c30AcAN-(1 -> 3)-beta-D-QuipNAc-(1 ->.
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页数:5
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