Characterization and immunolocalization of mutated ornithine decarboxylase antizyme from Angiostrongylus cantonensis

被引：1

作者：

Chen, Jing ^{[1
,2
]}

Liu, Qian ^{[1
,2
]}

Yang, Xiao ^{[1
,2
]}

Wu, Xiansheng ^{[1
,2
]}

Zhang, Dongjing ^{[1
,2
]}

He, Ai ^{[1
,2
]}

Zhan, Ximei ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Minist Educ, Key Lab Trop Dis Control, Guangzhou 510080, Guangdong, Peoples R China

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2013年 / 190卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Angiostrongylus cantonensis; Mutated ornithine decarboxylase antizyme; Clone; Cell proliferation; CELL-GROWTH; MOLECULAR CHARACTERIZATION; EXPRESSION; POLYAMINES; INHIBITOR; CARCINOGENESIS; PROLIFERATION; CONSERVATION; INDUCTION; TARGETS;

D O I：

10.1016/j.molbiopara.2013.06.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ornithine decarboxylase antizyme (OAZ), a prominent regulator of cell proliferation, DNA/RNA transformation and tumorigenesis, can bind to ornithine decarboxylase (ODC) and facilitate its degradation. Expression of OAZ requires a unique ribosomal frame shift that is regulated by levels of polyamine in the cell. In this study, we cloned an OAZ gene with the +1 ribosomal frame-shift from a fourth-stage larvae cDNA library of Angiostrongylus cantonensis. We removed one nucleotide to express the gene without polyamine. The sequence analysis showed that the deleted-mutation ornithine decarboxylase antizyme (DM-AcOAZ) contained a conservative domain related to other species OAZ. Quantitative real-time PCR revealed that DM-AcOAZ was expressed in L3 and L4 stages and adult female worms. More notably the expression level is the highest in the adult female stage. Immunohistochemical studies indicated that DM-AcOAZ was specifically localized in the uterus, oocyte and intestine in adult female worms. MTT assays showed that in DM-AcOAZ transfected HeLa cells, cell proliferation is inhibited. In conclusion, DM-AcOAZ may be a female-enriched protein and may involved in the cell proliferation in A. cantonensis. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：76 / 81

页数：6

共 26 条

[1] SPERMINE TOXICITY AND GLUTATHIONE DEPLETION IN BHK-21/C13 CELLS [J].