Cloning of a dextransucrase gene (fmcmds) from a constitutive dextransucrase hyper-producing Leuconostoc mesenteroides B-512FMCM developed using VUV

被引：16

作者：

Ryu, HJ

Kim, D ^{[1
]}

Kim, DW

Moon, YY

Robyt, JF

机构：

[1] Chonnam Natl Univ, Dept Fine Chem Engn, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Dept Biochem Engn, Kwangju 500757, South Korea

[3] Chonnam Natl Univ, Res Inst Catalysis, Kwangju 500757, South Korea

[4] Seoul Natl Univ, New Biomat Agr Res Ctr, Suwon 441744, South Korea

[5] Kangnung Natl Univ, Dept Phys, Kangnung 210702, South Korea

[6] Iowa State Univ, Dept Bot, Ames, IA 50011 USA

[7] Iowa State Univ, Lab Carbohydrate Chem & Enzymol, Ames, IA 50011 USA

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 05期

关键词：

dextransucrase; gene for dextransucrase; gene sequence; glycosyltransferase; Leuconostoc mesenteroides;

D O I：

10.1023/A:1005609718173

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dextransucrase (FMCMDS) from Leuconostoc mesenteroides B-512FMCM, a dextransucrase constitutive and hyper-producing strain, catalyzes the synthesis of dextran from sucrose. The coding region for fmcmds was isolated and sequenced. It consisted of an open reading frame (ORF) of 4699 bp, coding for a 1527 amino acid protein with a molecular mass of 170 kDa. However, it showed a dextransucrase activity band at 180 kDa in SDS-PAGE. Only one nucleotide changed in the promoter site and two amino acid residues were changed in the structural gene from that of the parent L. mesenteroides NRRL B-512F dsrS; an inducible dextransucrase gene of low productivity.

引用

页码：421 / 425

页数：5

共 14 条

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BHATNAGAR R, 1996, U81374 GENB

[2]

HAJAHMAD Y, 1994, BIOTECHNIQUES, V16, P390

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