Alleles of afd1 dissect REC8 functions during meiotic prophase I

被引:128
作者
Golubovskaya, Inna N.
Hamant, Olivier
Timofejeva, Ljuda
Wang, Chung-Ju Rachel
Braun, David
Meeley, Robert
Cande, W. Zacheus [1 ]
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] NI Vavilov Inst Plant Breeding, St Petersburg 190000, Russia
[3] Tallinn Univ Technol, Dept Gene Technol, EE-19086 Tallinn, Estonia
[4] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
[5] Pioneer HiBred Int Inc, Johnston, IA 50131 USA
关键词
meiosis; REC8; leptotene chromosome; axial element; RAD51; bouquet;
D O I
10.1242/jcs.03054
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
REC8 is a master regulator of chromatin structure and function during meiosis. Here, we dissected the functions of absence of first division (afd1), a maize rec8/alpha-kleisin homolog, using a unique afd1 allelic series. The first observable defect in afd1 mutants is the inability to make a leptotene chromosome. AFD1 protein is required for elongation of axial elements but not for their initial recruitment, thus showing that AFD1 acts downstream of ASY1/HOP1. AFD1 is associated with the axial and later the lateral elements of the synaptonemal complex. Rescuing 50% of axial element elongation in the weakest afd1 allele restored bouquet formation demonstrating that extent of telomere clustering depends on axial element elongation. However, rescuing bouquet formation was not sufficient for either proper RAD51 distribution or homologous pairing. It provides the basis for a model in which AFD1/REC8 controls homologous pairing through its role in axial element elongation and the subsequent distribution of the recombination machinery independent of bouquet formation.
引用
收藏
页码:3306 / 3315
页数:10
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