D-LL-31 in combination with ceftazidime synergistically enhances bactericidal activity and biofilm destruction in Burkholderia pseudomallei

被引：19

作者：

Wongkaewkhiaw, Saharut ^{[1
,2
]}

Taweechaisupapong, Suwimol ^{[2
,3
]}

Anutrakunchai, Chitchanok ^{[3
]}

Nazmi, Kamran ^{[4
,5
]}

Bolscher, Jan G. M. ^{[4
,5
]}

Wongratanacheewin, Surasakdi ^{[1
,2
]}

Kanthawong, Sakawrat ^{[1
,2
,3
]}

机构：

[1] Khon Kaen Univ, Dept Microbiol, Fac Med, Khon Kaen, Thailand

[2] Khon Kaen Univ, Melioidosis Res Ctr, Khon Kaen, Thailand

[3] Khon Kaen Univ, Biofilm Res Grp, Fac Dent, Khon Kaen, Thailand

[4] Univ Amsterdam, Acad Ctr Dent Amsterdam ACTA, Dept Oral Biochem, Amsterdam, Netherlands

[5] Vrije Univ Amsterdam, Amsterdam, Netherlands

来源：

BIOFOULING | 2019年 / 35卷 / 05期

关键词：

Antimicrobial activity; cathelicidin peptides; D-enantiomer peptide; bacterial biofilm; melioidosis; PSEUDOMONAS-AERUGINOSA; ANTIMICROBIAL PEPTIDES; STREPTOCOCCUS-MUTANS; ANTIBIOFILM ACTIVITY; TRUNCATED VARIANTS; LL-37; MELIOIDOSIS; LACTOFERRICIN; ANTIBIOTICS; RESISTANCE;

D O I：

10.1080/08927014.2019.1632835

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Melioidosis is a severe disease caused by Burkholderia pseudomallei. The biofilm of B. pseudomallei acquires resistance to several antibiotics and may be related to relapse in melioidosis patients. Here, the killing activity of antimicrobial peptides (LL-37, LL-31) and the D-enantiomers (D-LL-37, D-LL-31) in combination with ceftazidime (CAZ) against B. pseudomallei 1026b, H777 and a biofilm mutant M10, derived from H777 grown under biofilm-stimulating conditions was observed. Using static conditions, D-LL-31 exhibited the strongest killing activity against the three isolates in a dose-dependent manner. IC50 values for D-LL-31 ranged from 1 to 6 mu M, for isolates M10, H777, and 1026b, respectively. Moreover, D-LL-31 combined with CAZ synergistically decreased the IC50 values of the peptide and antibiotic and caused also disruption of biofilms of B. pseudomallei 1026b under flow conditions. Thus a combination of D-LL-31 and CAZ may enhance the efficacy of the currently used antibiotic treatments against B. pseudomallei.

引用

页码：573 / 584

页数：12

共 47 条

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[2]

[Anonymous], 2014, REV ANTIMICROBIAL RE

[3]

Anutrakunchai C, 2015, TROP BIOMED, V32, P300

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