Label-Free Highly Sensitive Detection of Proteins in Aqueous Solutions Using Surface-Enhanced Raman Scattering

被引：193

作者：

Han, Xiao X. ^{[1
,2
]}

Huang, Genin Gary ^{[2
]}

Zhao, Bing ^{[1
]}

Ozaki, Yukihiro ^{[2
]}

机构：

[1] Jilin Univ, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China

[2] Kwansei Gakuin Univ, Sch Sci & Technol, Dept Chem, Sanda, Hyogo 6691337, Japan

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 09期

关键词：

CYTOCHROME-C; SPECTROSCOPY; SILVER; ADSORPTION; MOLECULES; PEPTIDES; SERS;

D O I：

10.1021/ac900395x

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We detected concentration-dependent surface-enhanced Raman scattering (SERS) spectra of several label-free proteins (lysozyme, ribonuclease B, avidin, catalase, and hemoglobin) for the first time in aqueous solutions. Acidified sulfate was used as an aggregation agent to induce high electromagnetic enhancement in SERS. Strong SERS spectra of simple and conjugated protein samples could easily be accessed after the pretreatment with the aggregation agent. The detection limits of the proposed method for lysozyme and catalase were as low as 5 mu g/ mL and 50 ng/mL, respectively. This detection protocol for label-free proteins has combined simplicity, sensitivity, and reproducibility and allows routine qualitative and relatively quantitative detections. Thus, it has great potential in practical high-throughput protein detections.

引用

页码：3329 / 3333

页数：5

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