Mechanism of assembly of recombinant murine polyomavirus-like particles

被引：28

作者：

Schmidt, U ^{[1
]}

Rudolph, R ^{[1
]}

Böhm, G ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Inst Biotechnol, D-06120 Halle, Germany

来源：

JOURNAL OF VIROLOGY | 2000年 / 74卷 / 04期

关键词：

D O I：

10.1128/JVI.74.4.1658-1662.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

VP1 is the major viral coat protein of murine polyomavirus and can be used for the generation of virus-like particles in vitro. Here, me demonstrate that capsid assembly is an equilibrium reaction followed by oxidation of intracapsomere disulfide bonds, which are not essential for the formation of virus-like particles but enable complete particle assembly and prevent capsid dissembly.

引用

页码：1658 / 1662

页数：5

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