The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion

被引：32

作者：

Amer, AO

Valvano, MA ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Microbiol & Immunol, London, ON N6A 5C1, Canada

[2] Univ Western Ontario, Dept Med, London, ON N6A 5C1, Canada

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 02期

关键词：

D O I：

10.1128/JB.182.2.498-503.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.

引用

收藏

页码：498 / 503

页数：6

相关论文

共 39 条

[1] ROLE OF THE RFE GENE IN THE BIOSYNTHESIS OF THE ESCHERICHIA-COLI O7-SPECIFIC LIPOPOLYSACCHARIDE AND OTHER O-SPECIFIC POLYSACCHARIDES CONTAINING N-ACETYLGLUCOSAMINE [J].

ALEXANDER, DC ;

VALVANO, MA .

JOURNAL OF BACTERIOLOGY, 1994, 176 (22) :7079-7084

[2] PBLUESCRIPT-II - GENE-MAPPING VECTORS [J].

ALTINGMEES, MA ;

SHORT, JM .

NUCLEIC ACIDS RESEARCH, 1989, 17 (22) :9494-9494

[3]

AMER A, UNPUB

[4]

BARR K, 1987, J BIOL CHEM, V262, P7142

[5] ROLE OF RFE AND RFBF IN THE INITIATION OF BIOSYNTHESIS OF D-GALACTAN-I, THE LIPOPOLYSACCHARIDE O-ANTIGEN FROM KLEBSIELLA-PNEUMONIAE SEROTYPE-O1 [J].

CLARKE, BR ;

BRONNER, D ;

KEENLEYSIDE, WJ ;

SEVERN, WB ;

RICHARDS, JC ;

WHITFIELD, C .

JOURNAL OF BACTERIOLOGY, 1995, 177 (19) :5411-5418

[6] Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method [J].

Cserzo, M ;

Wallin, E ;

Simon, I ;

vonHeijne, G ;

Elofsson, A .

PROTEIN ENGINEERING, 1997, 10 (06) :673-676

[7] DIRECTIONALITY IN PROTEIN TRANSLOCATION ACROSS MEMBRANES - THE N-TAIL PHENOMENON [J].

DALBEY, RE ;

KUHN, A ;

VONHEIJNE, G .

TRENDS IN CELL BIOLOGY, 1995, 5 (10) :380-383

[8]

DALNOGARE AR, 1988, GLYCOBIOLOGY, V8, P625

[9] Hamster UDP-N-acetylglucosamine:dolichol-P N-acetylglucosamine-1-P transferase has multiple transmembrane spans and a critical cytosolic loop [J].

Dan, N ;

Middleton, RB ;

Lehrman, MA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (48) :30717-30724

[10] Oligomerization of hamster UDP-GlcNAc:Dolichol-P GlcNAc-1-P transferase, an enzyme with multiple transmembrane spans [J].

Dan, N ;

Lehrman, MA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (22) :14214-14219

← 1 2 3 4 →