Development of a live attenuated vaccine candidate against duck Tembusu viral disease

被引：47

作者：

Li, Guoxin ^{[1
]}

Gao, Xuyuan ^{[1
]}

Xiao, Yali ^{[1
]}

Liu, Shaoqiong ^{[1
]}

Peng, Shan ^{[1
]}

Li, Xuesong ^{[1
]}

Shi, Ying ^{[1
]}

Zhang, Yuee ^{[1
]}

Yu, Lei ^{[1
]}

Wu, Xiaogang ^{[1
]}

Yan, Pixi ^{[1
]}

Yan, Liping ^{[1
]}

Teng, Qiaoyang ^{[1
]}

Tong, Guangzhi ^{[1
]}

Li, Zejun ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Dept Avian Infect Dis, Shanghai 200241, Peoples R China

来源：

VIROLOGY | 2014年 / 450卷

基金：

中国国家自然科学基金;

关键词：

Flavivirus; Duck Tembusu virus; Attenuation; BORNE ENCEPHALITIS-VIRUS; AMINO-ACID SUBSTITUTION; JAPANESE-ENCEPHALITIS; MOUSE NEUROINVASIVENESS; NONCODING REGION; NY99; STRAIN; ENVELOPE; MUTATIONS; SA14-14-2; NEUROVIRULENCE;

D O I：

10.1016/j.virol.2013.12.028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5 log(10) tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5 log(10) TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5 log(10) TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：233 / 242

页数：10

共 49 条

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