Determination of the absolute configuration of selenomethionine from antarctic krill by RP-HPLC/ICP-MS using chiral derivatization agents

被引：24

作者：

Bergmann, J ^{[1
]}

Lassen, S ^{[1
]}

Prange, A ^{[1
]}

机构：

[1] GKSS Forschungszentrum Geesthacht GmbH, Inst Coastal Res, D-21502 Geesthacht, Germany

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2004年 / 378卷 / 06期

关键词：

selenomethionine; chiral speciation; enantiomer separation; inductively coupled plasma mass spectrometry krill;

D O I：

10.1007/s00216-003-2472-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast and sensitive method was developed for the determination of the absolute configuration of selenomethionine. The enantiomers of selenomethionine were converted into diastereomeric isoindole derivatives by reaction with o-phthaldialdehyde and N-isobutyryl-L-cysteine. This easy-to-handle reaction proceeds quantitatively in a few minutes at room temperature. Separation and detection of the diastereomers was achieved by reversed-phase high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (RP-HPLC/ICP-MS) using a conventional C18 reversed-phase column. Detection limits of about 4 mug L-1 were obtained. The method was applied to the determination of the configuration of selenomethionine extracted from antarctic krill, which turned out to possess the L-Configuration.

引用

页码：1624 / 1629

页数：6

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