DNA biosensor by self-assembly of carbon nanotubes and DNA to detect riboflavin

被引：18
|
作者：
Li, Jing [1 ]
Zhang, Yunhuai [1 ]
Yang, Tongyi [2 ]
Zhang, Huai [3 ]
Yang, Yixuan [4 ]
Xiao, Peng [5 ]
机构：
[1] Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China
[2] Nanjing Univ, Sch Life Sci, Nanjing 210093, Peoples R China
[3] Liming Res Inst Chem Ind, Luoyang 471001, Peoples R China
[4] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
[5] Chongqing Univ, Coll Math & Phys, Chongqing 400044, Peoples R China
来源：
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2009年 / 29卷 / 08期
关键词：
Self-assembly; Single-walled carbon nanotube; DNA; Riboflavin; Cyclic voltammetry; FLUORESCENCE DETECTION; MULTILAYER FILMS; NANOPARTICLES; FABRICATION; BINDING; CHEMILUMINESCENCE; ELECTRODE; ACID;
D O I：
10.1016/j.msec.2009.06.006
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
The fabrication of biosensors via self-assembly of single-walled carbon nanotubes (SWNTs) and DNA on a platinum electrode was presented in this paper. The carboxylic SWNTs were assembled on an amine-modified platinum electrode surface and followed by the assembly of NH2-DNA with the carboxyl-amine coupling. The decorated surface was characterized by Field Emission Electron Microscopy (FEG-SEM) and electrochemical experiments. which showed that the reaction of DNA-SWNTs biosensor was quasi-reversible. The mechanism of DNA and riboflavin (VB2) was studied by cyclic, voltammetry and UV-Vis spectroscopy. The fabricated SWNTs-reinforced biosensor exhibits high sensitivity and low detection limit for the tested VB2 compared to the reported methods. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：2360 / 2364
页数：5
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