eIF4A2 is a host factor required for efficient HIV-1 replication

被引：15

作者：

Ndzinu, Jerry Kwame ^{[1
]}

Takeuchi, Hiroaki ^{[1
]}

Saito, Hideki ^{[1
]}

Yoshida, Takeshi ^{[1
]}

Yamaoka, Shoji ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Dept Mol Virol, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138519, Japan

来源：

MICROBES AND INFECTION | 2018年 / 20卷 / 06期

关键词：

HIV-1; Host factor; eIF4A2; Viral cDNA synthesis; RNAi; DEAD-box protein; RNA HELICASE; TYPE-1; VIRUS; INITIATION; PROTEINS; SCREEN; CELLS; REV;

D O I：

10.1016/j.micinf.2018.05.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Host factors are required for efficient HIV-1 replication. To identify these factors, genome-wide RNA interference screening was performed using a human T cell line. In the present study, we assessed whether eukaryotic translation initiation factor 4A isoform 2 (eIF4A2), a DEAD-box protein identified in our screen, is necessary for efficient HIV-1 replication. Exploiting MT4C5 cells depleted of eIF4A2 by stable expression of eIF4A2-specific short-hairpin RNA (shRNA) using a lentiviral system, we found that depletion of eIF4A2 markedly inhibited the infection of a replication-competent reporter HIV-1. eIF4A2 depletion reduced the efficiency of viral cDNA synthesis with virion entry into target cells being unaffected. Depletion of eIF4A2 also inhibited HIV-1 spreading infection in a knockdown level-dependent manner. These results suggest that HIV-1 requires eIF4A2 for optimal replication in human T cells. (C) 2018 The Authors. Published by Elsevier Masson SAS on behalf of Institut Pasteur.

引用

页码：346 / 352

页数：7

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