Corneal epithelial stem cell delivery using cell sheet engineering: Not lost in transplantation

被引：35
作者：
Yang, Joseph
Yamato, Masayuki
Nishida, Kohji
Hayashida, Yasutaka
Shimizu, Tatsuya
Kikuchi, Akihiko
Tano, Yasuo
Okano, Teruo
机构：
[1] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo 1628666, Japan
[2] Tohoku Univ, Grad Sch Med, Dept Ophthalmol, Sendai, Miyagi 980, Japan
[3] Osaka Univ, Sch Med, Dept Ophthalmol, Osaka, Japan
来源：
JOURNAL OF DRUG TARGETING | 2006年 / 14卷 / 07期
基金：
日本科学技术振兴机构;
关键词：
cell-based therapy; corneal epithelial stem cells; cell sheet engineering; temperature-responsive culture dishes;
D O I：
10.1080/10611860600847997
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Cell-based therapies have now generated significant interest as novel drug delivery systems, with various adult cell types used in treating a wide range of diseases. To overcome the limits that restrict treatments for corneal surface dysfunction, corneal epithelial stem cells expanded ex vivo have been applied as an alternative approach. While previous studies used various carrier substrates, we present a novel method using cell sheet engineering with temperature-responsive culture dishes to create carrier-free corneal epithelial stem cell sheets that can be transplanted without sutures. Results from clinical trials reveal successful transplantation with the recovery of lost visual acuity in all cases. Cell sheet engineering, therefore, presents a novel method for the delivery of corneal epithelial stem cells, and can also be applied for other approaches of cellular therapeutics.
引用
收藏
页码：471 / 482
页数：12
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