Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase

被引：25

作者：

Wang, Dezheng ^{[1
]}

Wang, Cheng ^{[1
]}

Wu, Hui ^{[1
]}

Li, Zhimin ^{[1
,2
]}

Ye, Qin ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[2] Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2016年 / 43卷 / 01期

基金：

美国国家科学基金会;

关键词：

Glutathione; Escherichia coli; Bifunctional glutathione synthetase; One-strain system; Energy regeneration; GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; FED-BATCH CULTURE; HIGH CELL-DENSITY; SACCHAROMYCES-CEREVISIAE; CANDIDA-UTILIS; PROTEIN; OVERPRODUCTION; FERMENTATIONS; BIOSYNTHESIS; ACETATE;

D O I：

10.1007/s10295-015-1707-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glutathione (GSH) is an important bioactive substance applied widely in pharmaceutical and food industries. Due to the strong product inhibition in the GSH biosynthetic pathway, high levels of intracellular content, yield and productivity of GSH are difficult to achieve. Recently, a novel bifunctional GSH synthetase was identified to be less sensitive to GSH. A recombinant Escherichia coli strain expressing gshF encoding the bifunctional glutathione synthetase of Streptococcus thermophilus was constructed for GSH production. In this study, efficient GSH production using this engineered strain was investigated. The cultivation process was optimized by controlling dissolved oxygen (DO), amino acid addition and glucose feeding. 36.8 mM (11.3 g/L) GSH were formed at a productivity of 2.06 mM/h when the amino acid precursors (75 mM each) were added and glucose was supplied as the sole carbon and energy source.

引用

页码：45 / 53

页数：9

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