Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates

被引:146
作者
Lovseth, A [1 ]
Loncarevic, S [1 ]
Berdal, KG [1 ]
机构
[1] Natl Vet Inst, Sect Feed & Food Microbiol, N-0033 Oslo, Norway
关键词
D O I
10.1128/JCM.42.8.3869-3872.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.
引用
收藏
页码:3869 / 3872
页数:4
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