Determining cell-of-origin subtypes of diffuse large B-cell lymphoma using gene expression in formalin-fixed paraffin-embedded tissue

被引：487

作者：

Scott, David W. ^{[1
]}

Wright, George W. ^{[2
]}

Williams, P. Mickey ^{[3
]}

Lih, Chih-Jian ^{[3
]}

Walsh, William ^{[3
]}

Jaffe, Elaine S. ^{[4
]}

Rosenwald, Andreas ^{[5
]}

Campo, Elias ^{[6
]}

Chan, Wing C. ^{[7
]}

Connors, Joseph M. ^{[1
]}

Smeland, Erlend B. ^{[8
,9
,10
]}

Mottok, Anja ^{[1
]}

Braziel, Rita M. ^{[11
]}

Ott, German ^{[12
,13
]}

Delabie, Jan ^{[10
]}

Tubbs, Raymond R. ^{[14
]}

Cook, James R. ^{[15
]}

Weisenburger, Dennis D. ^{[16
]}

Greiner, Timothy C. ^{[7
]}

Glinsmann-Gibson, Betty J. ^{[17
]}

Fu, Kai ^{[7
]}

Staudt, Louis M. ^{[18
]}

Gascoyne, Randy D. ^{[1
,19
]}

Rimsza, Lisa M. ^{[17
]}

机构：

[1] British Columbia Canc Agcy, Ctr Lymphoid Canc, Vancouver, BC V5Z 4E6, Canada

[2] NCI, Biometr Res Branch, Div Canc Treatment & Diag, Bethesda, MD 20892 USA

[3] Leidos Med Res Inc, Mol Characterizat Lab, Frederick Natl Lab Canc Res, Frederick, MD USA

[4] NCI, Pathol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

[5] Univ Wurzburg, Inst Pathol, Wurzburg, Germany

[6] Hosp Clin Barcelona, Dept Pathol, Barcelona, Spain

[7] Univ Nebraska Med Ctr, Dept Pathol & Microbiol, Omaha, NE USA

[8] Univ Oslo, Dept Immunol, Oslo, Norway

[9] Univ Oslo, Ctr Canc Biomed, Oslo, Norway

[10] Oslo Univ Hosp, Oslo, Norway

[11] Oregon Hlth & Sci Univ, Dept Pathol, Portland, OR 97201 USA

[12] Robert Bosch Krankenhaus, Dept Clin Pathol, Stuttgart, Germany

[13] Dr Margarete Fischer Bosch Inst Clin Pharmacol, Stuttgart, Germany

[14] Cleveland Clin Fdn, Cleveland, OH 44195 USA

[15] Cleveland Clin, Dept Mol Pathol & Lab Med, Cleveland, OH 44106 USA

[16] City Hope Natl Med Ctr, Dept Pathol, Duarte, CA USA

[17] Univ Arizona, Dept Pathol, Tucson, AZ USA

[18] NCI, Metab Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

[19] British Columbia Canc Agcy, Dept Pathol, Vancouver, BC V5Z 4E6, Canada

来源：

BLOOD | 2014年 / 123卷 / 08期

基金：

加拿大健康研究院;

关键词：

MOLECULAR CLASSIFICATION; SURVIVAL; PREDICTS; CHEMOTHERAPY; MICROARRAY; MODEL;

D O I：

10.1182/blood-2013-11-536433

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The assignment of diffuse large B-cell lymphoma into cell-of-origin (COO) groups is becoming increasingly important with the emergence of novel therapies that have selective biological activity in germinal center B cell-like or activated B cell-like groups. The Lymphoma/Leukemia Molecular Profiling Project's Lymph2Cx assay is a parsimonious digital gene expression (NanoString)-based test for COO assignment in formalin-fixed paraffin-embedded tissue (FFPET). The 20-gene assay was trained using 51 FFPET biopsies; the locked assay was then validated using an independent cohort of 68 FFPET biopsies. Comparisons were made with COO assignment using the original COO model on matched frozen tissue. In the validation cohort, the assay was accurate, with only 1 case with definitive COO being incorrectly assigned, and robust, with >95% concordance of COO assignment between 2 independent laboratories. These qualities, along with the rapid turnaround time, make Lymph2Cx attractive for implementation in clinical trials and, ultimately, patient management.

引用

页码：1214 / 1217

页数：4

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