Investigation of the interaction between five alkaloids and human hemoglobin by fluorescence spectroscopy and molecular modeling

被引：32

作者：

He, Wu ^{[1
]}

Dou, Huanjing ^{[1
]}

Li, Zhigang ^{[1
]}

Wang, Xiaogai ^{[1
]}

Wang, Lvjing ^{[1
]}

Wang, Ruiyong ^{[1
]}

Chang, Junbiao ^{[1
]}

机构：

[1] Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2014年 / 123卷

基金：

中国国家自然科学基金;

关键词：

Alkaloid; Human hemoglobin; Fluorescence spectroscopy; Molecular modeling; SERUM-ALBUMIN; BINDING; EMISSION; CAFFEINE; PROBE;

D O I：

10.1016/j.saa.2013.12.059

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

This work studied the interaction of human hemoglobin (HHb) with aminophylline, acefylline, caffeine, theophylline and diprophylline systematically by UV-vis absorption spectroscopy and fluorescence spectroscopy in combination with molecular modeling. Five alkaloids caused the fluorescence quenching of HHB by the formation of alkaloids-HHb complex. The binding constants and thermodynamic parameters were obtained. The hydrophobic and electrostatic interactions were the predominant intermolecular forces to stabilize these complexes. Results of thermodynamic analysis and molecular modeling showed that aminophylline was the strongest quencher and diprophylline was the weakest quencher. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：176 / 186

页数：11

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