Convalescent plasma anti SARS-CoV-2 spike protein ectodomain and receptor-binding domain IgG correlate with virus neutralization

被引：122

作者：

Salazar, Eric ^{[1
,2
]}

Kuchipudi, Suresh V. ^{[3
,4
]}

Christensen, Paul A. ^{[1
]}

Eagar, Todd ^{[1
,2
]}

Yi, Xin ^{[1
,2
]}

Zhao, Picheng ^{[1
]}

Jin, Zhicheng ^{[1
]}

Long, S. Wesley ^{[1
,2
,5
]}

Olsen, Randall J. ^{[1
,2
,5
]}

Chen, Jian ^{[1
,2
]}

Castillo, Brian ^{[1
,2
]}

Leveque, Christopher ^{[1
,2
]}

Towers, Dalton ^{[6
]}

Lavinder, Jason ^{[6
]}

Gollihar, Jimmy ^{[7
]}

Cardona, Jose ^{[7
]}

Ippolito, Gregory ^{[6
,8
]}

Nissly, Ruth ^{[3
]}

Bird, Ian ^{[3
]}

Greenawalt, Denver ^{[3
]}

Rossi, Randall M. ^{[9
]}

Gontu, Abhinay ^{[3
]}

Srinivasan, Sreenidhi ^{[9
]}

Poojary, Indira ^{[9
]}

Cattadori, Isabella M. ^{[4
,5
,10
]}

Hudson, Peter J. ^{[4
,9
,10
]}

Josleyn, Nicole M. ^{[11
]}

Prugar, Laura ^{[11
]}

Huie, Kathleen ^{[11
]}

Herbert, Andrew ^{[11
]}

Bernard, David W. ^{[1
,2
]}

Dye, John M. ^{[11
]}

Kapur, Vivek ^{[4
,9
,12
]}

Musser, James M. ^{[1
,2
,5
]}

机构：

[1] Houston Methodist Hosp, Dept Pathol & Genom Med, Houston, TX 77030 USA

[2] Weill Cornell Med Coll, Dept Pathol & Lab Med, New York, NY USA

[3] Penn State Univ, Penn State Anim Diagnost Lab, Dept Vet & Biomed Sci, University Pk, PA 16802 USA

[4] Penn State Univ, Ctr Infect Dis Dynam, University Pk, PA 16802 USA

[5] Houston Methodist Res Inst, Ctr Mol & Translat Human Infect Dis, Houston, TX USA

[6] Univ Texas Austin, Dept Mol Biosci, Austin, TX 78712 USA

[7] Univ Texas Austin, Combat Capabil Dev Command Army Res Lab South, Austin, TX 78712 USA

[8] Univ Texas Austin, Dept Oncol, Dell Med Sch, Austin, TX 78712 USA

[9] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA

[10] Penn State Univ, Dept Biol, University Pk, PA 16802 USA

[11] US Army, Med Res Inst Infect Dis, Frederick, MD USA

[12] Penn State Univ, Dept Anim Sci, University Pk, PA 16802 USA

来源：

JOURNAL OF CLINICAL INVESTIGATION | 2020年 / 130卷 / 12期

关键词：

HUMAN MONOCLONAL-ANTIBODY; SARS CORONAVIRUS; MAB;

D O I：

10.1172/JCI141206

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlights the urgent need for assays that detect protective levels of neutralizing antibodies. We studied the relationship among anti-spike ectodomain (anti-ECD), anti-receptor-binding domain (anti-RBD) IgG titers, and SARS-CoV-2 virus neutralization (VN) titers generated by 2 in vitro assays using convalescent plasma samples from 68 patients with COVID-19. We report a strong positive correlation between both plasma anti-RBD and anti-ECD IgG titers and in vitro VN titers. The probability of a VN titer of >= 160, the FDA-recommended level for convalescent plasma used for COVID-19 treatment, was >= 80% when anti-RBD or anti-ECD titers were >= 1:1350. Of all donors, 37% lacked VN titers of >= 160. Dyspnea, hospitalization, and disease severity were significantly associated with higher VN titer. Frequent donation of convalescent plasma did not significantly decrease VN or IgG titers. Analysis of 2814 asymptomatic adults found 73 individuals with anti-ECD IgG titers of >= 1:50 and strong positive correlation with anti-RBD and VN titers. Fourteen of these individuals had VN titers of >= 1:160, and all of them had anti-RBD titers of >= 1:1350. We conclude that anti-RBD or anti-ECD IgG titers can serve as a surrogate for VN titers to identify suitable plasma donors. Plasma anti-RBD or anti-ECD titers of >= 1:1350 may provide critical information about protection against COVID-19 disease.

引用

页码：6728 / 6738

页数：11

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