Antibacterial activity of Thymus vulgaris essential oil alone and in combination with cefotaxime against blaESBL producing multidrug resistant Enterobacteriaceae isolates

被引：56

作者：

Benameur, Qada ^{[1
,2
]}

Gervasi, Teresa ^{[3
]}

Pellizzeri, Vito ^{[3
]}

Pluchtova, Maria ^{[4
]}

Tali-Maama, Hassiba ^{[5
]}

Assaous, Farida ^{[5
]}

Guettou, Badia ^{[5
]}

Rahal, Kheira ^{[5
]}

Grulova, Daniela ^{[4
]}

Dugo, Giacomo ^{[3
,6
]}

Marino, Andreana ^{[7
]}

Ben-Mahdi, Meriem-Hind ^{[2
,8
]}

机构：

[1] Univ Abdelhamid Ibn Badis Mostaganem, Fac Nat Sci & Life, Dept Nursing, Mostaganem, Algeria

[2] Ecole Natl Super Vet El Harrach, Lab Rech Sante & Prod Anim, Algiers, Algeria

[3] Univ Messina, Dept Biomed & Dent Sci & Morphofunct Imaging, Messina, Italy

[4] Univ Presov, Dept Ecol, Fac Humanities & Nat Sci, Presov, Slovakia

[5] Inst Pasteur, Lab Bacteriol Med, Algiers, Algeria

[6] Univ Messina, Science4Life, Spin Off Co, Messina, Italy

[7] Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Messina, Italy

[8] Ecole Super Sci Aliments & Ind Agroalimentaires, Algiers, Algeria

来源：

NATURAL PRODUCT RESEARCH | 2019年 / 33卷 / 18期

关键词：

Thymus vulgaris; essential oil; antibacterial-resistance; Enterobacteriaceae; ESBL; synergism; SPECTRUM BETA-LACTAMASES; ESCHERICHIA-COLI; ANTIMICROBIAL ACTIVITY; ANTIBIOTIC-RESISTANCE; CHEMICAL-COMPOSITION; CLASSIFICATION;

D O I：

10.1080/14786419.2018.1466124

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim was to evaluate the susceptibility of bla(ESBL) producing Enterobacteriaceae to Slovakian Thymus vulgaris essential oil (TVEO) alone and in combination with cefotaxime (CTX). TVEO composition was determined by gas chromatograph-mass spectrometer (GC/MS). Susceptibility to 21 antibiotics was determined by disc diffusion assay. Genes characterization for resistance to beta-lactams was accomplished by polymerase chain reaction (PCR). The antibacterial activity was investigated by standard methods. The synergistic interaction was determined by checkerboard test. Thymol (34.5%), p-cymene (22.27%) and linalool (5.35%) were the major components present in the TVEO. The identified strains were multi-drug resistant (MDR). TVEO showed high activity against all MDR strains, including bla(ESBL) producing isolates, with inhibition zones and MIC values in the range of 24-40 mm/10 mu L and 2.87-11.5 mu g/mL, respectively. TVEO in combination with CTX showed a synergistic action against bla(SHV-12) producing Escherichia coli (FICI 0.28) and an additive effect vs ESBL producing Enterobacter cloacae (FICI 0.987). [GRAPHICS] .

引用

页码：2647 / 2654

页数：8

共 38 条

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