Presence of human herpesvirus 8 is specific to Kaposi's sarcoma lesion

被引：0

作者：

Lash, A

Rabkin, C

EmmertBuck, M

Merino, MJ

Liotta, L

Zhuang, ZP

机构：

[1] NCI,PATHOL LAB,NIH,BETHESDA,MD 20892

[2] NCI,VIRAL EPIDEMIOL BRANCH,NIH,BETHESDA,MD 20892

来源：

MOLECULAR DIAGNOSIS | 1997年 / 2卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Kaposi's sarcoma-associated herpesvirus; Kaposi's sarcoma; molecular pathology;

D O I：

10.1016/S1084-8592(97)80004-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Human herpesvirus 8 (HHV8) has previously been shown to be associated with Kaposi's sarcoma (KS) skin lesions in HIV-positive and negative patients. This study was designed to test, on a microscopic level, using the microdissection technique in frozen and formalin-fixed tissue, whether HHV8 DNA is specific to the KS lesion. Methods and Results: Thirty-seven evaluable KS lesions were microdissected and DNA extracted, and HHV8-specific polymerase chain reaction (PCR) was performed. All of the KS lesions showed a PCR product; none of the microscopically adjacent normal tissue samples showed a PCR product. One of the PCR products was sequenced, confirming the presence of HHV8 DNA. Conclusions: HHV8 was shown to be specific to the KS lesion at the microscopic level.

引用

页码：11 / 14

页数：4

共 11 条

[1] KAPOSIS SARCOMA-ASSOCIATED HERPESVIRUS INFECTS ENDOTHELIAL AND SPINDLE CELLS [J].