Quantification of Myocardial Perfusion Defect Size in Rats: Comparison between Quantitative Perfusion SPECT and Autoradiography

被引:3
作者
Wakabayashi, Hiroshi [1 ]
Taki, Junichi [1 ]
Inaki, Anri [1 ]
Hiromasa, Tomo [1 ]
Okuda, Koichi [2 ]
Shibutani, Takayuki [3 ]
Shiba, Kazuhiro [4 ]
Kinuya, Seigo [1 ]
机构
[1] Kanazawa Univ Hosp, Dept Nucl Med, 13-1 Takara Machi, Kanazawa, Ishikawa 9208641, Japan
[2] Kanazawa Med Univ, Dept Phys, Kaho Ku, 1-1 Daigaku, Uchinada, Ishikawa 9200293, Japan
[3] Kanazawa Univ, Inst Med Pharmaceut & Hlth Sci, Dept Quantum Med Technol, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 9200942, Japan
[4] Kanazawa Univ, Div Tracer Kinet, Adv Sci Res Ctr, 13-1 Takara Machi, Kanazawa, Ishikawa 9208641, Japan
关键词
Tc-99m]MIBI; Myocardial perfusion; Myocardial infarction; SPECT; Autoradiography; EMISSION COMPUTED-TOMOGRAPHY; EJECTION FRACTION MEASUREMENTS; PINHOLE GATED SPECT; PROGNOSTIC VALUE; CARDIAC DEATH; ISCHEMIA; REPERFUSION; INFARCTION; REPRODUCIBILITY; STRATIFICATION;
D O I
10.1007/s11307-018-1159-1
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Ultra-high resolution single-photon emission computed tomography (SPECT) system, using multiple pinhole collimators, has been applied to the imaging of small rodents. We aimed to compare the myocardial infarction (MI) area on quantitative perfusion single-photon emission computed tomography (QPS; Cedars-Sinai Medical Center, USA) with that on high-resolution autoradiography in rat model to determine the accuracy of perfusion defect measurement by QPS. After thoracotomy, rats (n = 9) had their left coronary arteries occluded and reperfused before injection with 185 MBq [Tc-99m] methoxyisobutylisonitrile ([Tc-99m]MIBI) for SPECT and autoradiography. Healthy rats (n = 28) were similarly scanned to create a normal database on which to base QPS. The MI area on SPECT images was analysed automatically by QPS software. For the autoradiography images, regions of interest for MI were set at 1 mm intervals. In normal rats, [Tc-99m]MIBI accumulated throughout the left ventricles, and a polar map of ventricular perfusion showed the lowest and highest uptakes in the inferior (68 % +/- 4 %) and anterior (92 % +/- 5 %) walls, respectively. In the rat MI model, the percentage of polar map with reduced [Tc-99m]MIBI uptake correlated strongly with the percentage of left ventricle with MI on autoradiography (r (2) = 0.90). QPS can quantitatively evaluate MI severity on myocardial perfusion images in rats, with comparable results to autoradiography. This widely available software could promote the development of new techniques for analysing cardiac images in small animals.
引用
收藏
页码:544 / 550
页数:7
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