Role of protein kinase A in regulating steroid sulfate uptake for estrogen production in human placental choriocarcinoma cells

被引：9
作者：
Tomi, M. [1 ]
Miyata, Y. [1 ]
Noguchi, S. [1 ]
Nishimura, S. [1 ]
Nishimura, T. [1 ]
Nakashima, E. [1 ]
机构：
[1] Keio Univ, Fac Pharm, Minato Ku, Tokyo 1058512, Japan
来源：
PLACENTA | 2014年 / 35卷 / 08期
关键词：
OAT4; Estrogen synthesis; Protein kinase A; Dehydroepiandrosterone sulfate; Syncytiotrophoblast; HUMAN-PREGNANCY; BIOSYNTHESIS; EXPRESSION; TRANSPORTERS; AROMATASE; JEG-3;
D O I：
10.1016/j.placenta.2014.06.003
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The purpose of this study is to assess the role of the protein kinase A (PKA) in regulating uptake of dehydroepiandrosterone sulfate (DHEAS), an estrogen precursor, by syncytiotrophoblasts. Forskolin, a PKA activator, significantly increased [H-3]DHEAS uptake and the mRNA expression levels of organic anion transporter (OAT) 4 and CYP19A1 in choriocarcinoma JEG-3 cells, while other steroid sulfate transporters present in the placenta showed no change in expression level. KT5720, a PKA inhibitor, attenuated these effects of forskolin. Accordingly, the PKA pathway appears to play an important role in estrogen synthesis by cooperatively regulating OAT4 and steroidogenic enzymes in syncytiotrophoblasts. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：658 / 660
页数：3
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