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Magnolol Reduced TNF-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Endothelial Cells via JNK/p38 and NF-κB Signaling Pathways
被引:31
作者:
Liang, Chan-Jung
[1
,2
]
Lee, Chiang-Wen
[2
,3
]
Sung, Hsin-Ching
[1
,4
]
Chen, Yung-Hsiang
[5
]
Wang, Shu-Huei
[1
]
Wu, Pei-Jhen
[1
]
Chiang, Yao-Chang
[7
]
Tsai, Jaw-Shiun
[8
]
Wu, Chau-Chung
[9
]
Li, Chi-Yuan
[6
]
Chen, Yuh-Lien
[1
]
机构:
[1] Natl Taiwan Univ, Dept Anat & Cell Biol, Taipei 100, Taiwan
[2] Chang Gung Univ Sci & Technol, Res Ctr Ind Human Ecol, Taoyuan, Taiwan
[3] Chang Gung Univ Sci & Technol, Div Basic Med Sci, Dept Nursing, Chiayi, Taiwan
[4] Chang Gung Univ, Dept Anat, Coll Med, Taoyuan, Taiwan
[5] China Med Univ, Grad Inst Integrated Med, Taichung, Taiwan
[6] China Med Univ, Inst Clin Med Sci, Taichung, Taiwan
[7] China Med Univ Hosp, Ctr Drug Abuse & Addict, Taichung, Taiwan
[8] Natl Taiwan Univ Hosp, Dept Family Med, Taipei, Taiwan
[9] Natl Taiwan Univ Hosp, Dept Internal Med & Primary Care Med, Taipei, Taiwan
来源:
AMERICAN JOURNAL OF CHINESE MEDICINE
|
2014年
/
42卷
/
03期
关键词:
Magnolol;
Vascular Cell Adhesion Molecule-1;
Mitogen-Activated Protein Kinase (MAPKs);
Transcriptional Factors;
Endothelial Cells;
INDUCED INFLAMMATORY RESPONSE;
IN-VITRO;
MECHANISMS;
ATHEROSCLEROSIS;
ANTIOXIDANT;
ACTIVATION;
DISEASE;
VCAM-1;
INJURY;
AORTA;
D O I:
10.1142/S0192415X14500402
中图分类号:
R [医药、卫生];
学科分类号:
10 ;
摘要:
Expression of cell adhesion molecules by the endothelium and the attachment of leukocytes to these cells play major roles in inflammation and cardiovascular disorders. Magnolol, a major active component of Magnolia officinalis, has antioxidative and anti-inflammatory properties. In the present study, the effects of magnolol on the expression of vascular cell adhesion molecule-1 (VCAM-1) in human aortic endothelial cells (HAECs) and the related mechanisms were investigated. TNF-alpha induced VCAM-1 protein expression and mRNA stability were significantly decreased in HAECs pre-treated with magnolol. Magnolol significantly reduced the phosphorylation of ERK, JNK, and p38 in TNF-alpha-treated HAECs. The decrease in VCAM-1 expression in response to TNF-alpha treatment was affected by JNK and p38 inhibitors, not by an ERK inhibitor. Magnolol also attenuates NF-kappa B activation and the translocation of HuR (an RNA binding protein) in TNF-alpha-stimulated HAECs. The VCAM-1 expression was weaker in the aortas of TNF-alpha-treated apo-E deficient mice with magnolol treatment. These data demonstrate that magnolol inhibits TNF-alpha-induced JNK/p38 phosphorylation, HuR translocation, NF-kappa B activation, and thereby suppresses VCAM-1 expression resulting in reduced leukocyte adhesion. Taken together, these results suggest that magnolol has an anti-inflammatory property and may play an important role in the prevention of atherosclerosis and inflammatory responses.
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页码:619 / 637
页数:19
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