Magnolol Reduced TNF-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Endothelial Cells via JNK/p38 and NF-κB Signaling Pathways

被引:31
作者
Liang, Chan-Jung [1 ,2 ]
Lee, Chiang-Wen [2 ,3 ]
Sung, Hsin-Ching [1 ,4 ]
Chen, Yung-Hsiang [5 ]
Wang, Shu-Huei [1 ]
Wu, Pei-Jhen [1 ]
Chiang, Yao-Chang [7 ]
Tsai, Jaw-Shiun [8 ]
Wu, Chau-Chung [9 ]
Li, Chi-Yuan [6 ]
Chen, Yuh-Lien [1 ]
机构
[1] Natl Taiwan Univ, Dept Anat & Cell Biol, Taipei 100, Taiwan
[2] Chang Gung Univ Sci & Technol, Res Ctr Ind Human Ecol, Taoyuan, Taiwan
[3] Chang Gung Univ Sci & Technol, Div Basic Med Sci, Dept Nursing, Chiayi, Taiwan
[4] Chang Gung Univ, Dept Anat, Coll Med, Taoyuan, Taiwan
[5] China Med Univ, Grad Inst Integrated Med, Taichung, Taiwan
[6] China Med Univ, Inst Clin Med Sci, Taichung, Taiwan
[7] China Med Univ Hosp, Ctr Drug Abuse & Addict, Taichung, Taiwan
[8] Natl Taiwan Univ Hosp, Dept Family Med, Taipei, Taiwan
[9] Natl Taiwan Univ Hosp, Dept Internal Med & Primary Care Med, Taipei, Taiwan
来源
AMERICAN JOURNAL OF CHINESE MEDICINE | 2014年 / 42卷 / 03期
关键词
Magnolol; Vascular Cell Adhesion Molecule-1; Mitogen-Activated Protein Kinase (MAPKs); Transcriptional Factors; Endothelial Cells; INDUCED INFLAMMATORY RESPONSE; IN-VITRO; MECHANISMS; ATHEROSCLEROSIS; ANTIOXIDANT; ACTIVATION; DISEASE; VCAM-1; INJURY; AORTA;
D O I
10.1142/S0192415X14500402
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Expression of cell adhesion molecules by the endothelium and the attachment of leukocytes to these cells play major roles in inflammation and cardiovascular disorders. Magnolol, a major active component of Magnolia officinalis, has antioxidative and anti-inflammatory properties. In the present study, the effects of magnolol on the expression of vascular cell adhesion molecule-1 (VCAM-1) in human aortic endothelial cells (HAECs) and the related mechanisms were investigated. TNF-alpha induced VCAM-1 protein expression and mRNA stability were significantly decreased in HAECs pre-treated with magnolol. Magnolol significantly reduced the phosphorylation of ERK, JNK, and p38 in TNF-alpha-treated HAECs. The decrease in VCAM-1 expression in response to TNF-alpha treatment was affected by JNK and p38 inhibitors, not by an ERK inhibitor. Magnolol also attenuates NF-kappa B activation and the translocation of HuR (an RNA binding protein) in TNF-alpha-stimulated HAECs. The VCAM-1 expression was weaker in the aortas of TNF-alpha-treated apo-E deficient mice with magnolol treatment. These data demonstrate that magnolol inhibits TNF-alpha-induced JNK/p38 phosphorylation, HuR translocation, NF-kappa B activation, and thereby suppresses VCAM-1 expression resulting in reduced leukocyte adhesion. Taken together, these results suggest that magnolol has an anti-inflammatory property and may play an important role in the prevention of atherosclerosis and inflammatory responses.
引用
收藏
页码:619 / 637
页数:19
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[41]   Antioxidants inhibit the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 induced by oxidized LDL on human umbilical vein endothelial cells [J].
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[48]   Factors influencing the ability of HDL to inhibit expression of vascular cell adhesion molecule-1 in endothelial cells [J].
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