In Vivo Immunomodulatory Potential of Partial Purified Lectin from the Saffron Milk Cap Mushroom, Lactarius deliciosus (Agaricomycetes), against Colloidal Carbon Particles

被引：3

作者：

Esseddik, Toumi Mohammed ^{[1
]}

Redouane, Rebai ^{[2
,3
]}

Farouk, Kebaili Fethi ^{[1
]}

Fateh, Merouane ^{[2
]}

Khaled, Boulahrouf ^{[1
]}

Laid, Bahri ^{[1
]}

Perduca, Massimiliano ^{[4
]}

Youcef, Necib ^{[1
]}

机构：

[1] Univ Mentouri Bros Constantine 1, Fac Nat & Life Sci, Lab Microbiol Engn & Applicat, Dept Biochem & Mol & Cellular Biol, POB 325 Ain El Bey Way, Constantine 25017, Algeria

[2] Univ Town, Natl Higher Sch Biotechnol, Lab Biotechnol, BP E66 25100, Constantine, Algeria

[3] Univ Mohamed Kheider, Biskra, Algeria

[4] Univ Verona, Biocrystallog & Nanostruct Lab, Fac Biotechnol, Ca Vignal 1,Str Grazie 15, I-37134 Verona, Italy

来源：

INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS | 2020年 / 22卷 / 11期

关键词：

lectin; Lactarius deliciosus; phagocytic activity; half-life of carbon particles; carbon clearance; reticulo-endothelial system; medicinal mushrooms;

D O I：

10.1615/IntJMedMushrooms.2020036384

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mushroom compounds and biomolecules are known for their biological beneficial effects and dietary properties. Their molecules can be used in immunology for their ability to stimulate immune cells and in biotherapy of diseases. In this study, the immunomodulatory effect using carbon clearance test in vivo of partial purified lectin of Lactarius deliciosus using DEAE-Sephacyl column, with sugar affinity against galactose, methyl-beta-D-galactopyranoside and lactose, showed a significant effect on phagocytic activity and half-life of carbon particles in mice with different concentrations (5, 10, 15, and 30 mg/kg). The results showed that the immunomodulatory effect increased in low doses and decreased in high doses compared with the control group p < 0.0001. L. deliciosus lectin exerted a dose-dependent immunostimulant activity toward the reticulo-endothelial system, and phagocytic activity toward macrophages and neutrophils in spleen and liver against the colloidal carbon.

引用

页码：1043 / 1055

页数：13

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