A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA

被引：309

作者：

Fu, Xiuli ^{[1
]}

Cheng, Ziyi ^{[1
]}

Yu, Jimin ^{[1
]}

Choo, Priscilla ^{[2
]}

Chen, Lingxin ^{[3
]}

Choo, Jaebum ^{[1
]}

机构：

[1] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea

[2] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[3] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2016年 / 78卷

基金：

中国国家自然科学基金; 新加坡国家研究基金会;

关键词：

Human immunodeficiency virus type 1 (HIV-1); Surface-enhanced Raman scattering; Gold nanoparticles; DNA; Lateral flow assay; SURFACE-ENHANCED RAMAN; DETECTION PLATFORM; VISUAL DETECTION; STRIP BIOSENSOR; IMMUNOASSAY; NANOPARTICLES; SILVER; ELISA;

D O I：

10.1016/j.bios.2015.11.099

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

User-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low concentration range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：530 / 537

页数：8

共 37 条

[31] HOW DOES HIV CAUSE AIDS [J].

WEISS, RA .

SCIENCE, 1993, 260 (5112) :1273-1279

[32] A simple and universal "turn-on" detection platform for proteases based on surface enhanced Raman scattering (SERS) [J].

Wu, Zitong ;

Liu, Yifei ;

Liu, Yizhen ;

Xiao, Huaming ;

Shen, Aiguo ;

Zhou, Xiaodong ;

Hu, Jiming .

BIOSENSORS & BIOELECTRONICS, 2015, 65 :375-381

[33] A "turn-off' SERS-based detection platform for ultrasensitive detection of thrombin based on, enzymatic assays [J].

Wu, Zitong ;

Liu, Yizhen ;

Zhou, Xiaodong ;

Shen, Aiguo ;

Hu, Jiming .

BIOSENSORS & BIOELECTRONICS, 2013, 44 :10-15

[34] Aptamer-Functionalized Gold Nanoparticles as Probes in a Dry-Reagent Strip Biosensor for Protein Analysis [J].

Xu, Hui ;

Mao, Xun ;

Zeng, Qingxiang ;

Wang, Shengfu ;

Kawde, Abdel-Nasser ;

Liu, Guodong .

ANALYTICAL CHEMISTRY, 2009, 81 (02) :669-675

[35] Highly sensitive detection of thrombin using SERS-based magnetic aptasensors [J].

Yoon, Jiyeon ;

Choi, Namhyun ;

Ko, Juhui ;

Kim, Kihyung ;

Lee, Sangyeop ;

Choo, Jaebum .

BIOSENSORS & BIOELECTRONICS, 2013, 47 :62-67

[36] Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general [J].

Zhang, Shiyun ;

Garcia-D'Angeli, Alexa ;

Brennan, Joseph P. ;

Huo, Qun .

ANALYST, 2014, 139 (02) :439-445

[37] Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum [J].

Zhao, Peng ;

Wu, Yuanyuan ;

Zhu, Yihua ;

Yang, Xiaoling ;

Jiang, Xin ;

Xiao, Jingfan ;

Zhang, Yuanxing ;

Li, Chunzhong .

NANOSCALE, 2014, 6 (07) :3804-3809

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