Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots

被引：141

作者：

Ong, Wee Kiat ^{[1
]}

Tan, Chuen Seng ^{[2
]}

Chan, Kai Li ^{[1
]}

Goesantoso, Grace Gandi ^{[1
]}

Chan, Xin Hui Derryn ^{[1
]}

Chan, Edmund ^{[3
]}

Yin, Jocelyn ^{[3
]}

Yeo, Chia Rou ^{[3
]}

Khoo, Chin Meng ^{[3
]}

So, Jimmy Bok Yan

Shabbir, Asim ^{[4
]}

Toh, Sue-Anne ^{[3
]}

Han, Weiping ^{[5
,6
,7
]}

Sugii, Shigeki ^{[1
,5
]}

机构：

[1] ASTAR, Singapore Bioimaging Consortium, Lab Metab Med, Fat Metab & Stem Cell Grp, Singapore 138667, Singapore

[2] Natl Univ Singapore, Saw Swee Hock Sch Publ Hlth, Singapore 117597, Singapore

[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Med, Singapore 117599, Singapore

[4] Natl Univ Singapore Hosp, Dept Surg, Singapore 119074, Singapore

[5] Duke NUS Grad Med Sch, Cardiovasc & Metab Disorders Program, Singapore 169857, Singapore

[6] ASTAR, Singapore Bioimaging Consortium, Lab Metab Med, Singapore 138667, Singapore

[7] ASTAR, Inst Mol & Cell Biol, Metab Human Dis Unit, Singapore 138667, Singapore

来源：

STEM CELL REPORTS | 2014年 / 2卷 / 02期

基金：

英国医学研究理事会;

关键词：

INTERNATIONAL-SOCIETY; STROMAL/STEM CELLS; TISSUE; THERAPY; STATEMENT;

D O I：

10.1016/j.stemcr.2014.01.002

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.

引用

页码：171 / 179

页数：9

共 21 条

[1] The CD10 Enzyme Is a Key Player to Identify and Regulate Human Mammary Stem Cells [J].

Bachelard-Cascales, Elodie ;

Chapellier, Marion ;

Delay, Emmanuel ;

Pochon, Gaetan ;

Voeltzel, Thibault ;

Puisieux, Alain ;

de Fromentel, Claude Caron ;

Maguer-Satta, Veronique .

STEM CELLS, 2010, 28 (06) :1081-1088

[2] Comprehensive Phenotypic Characterization of Human Adipose-Derived Stromal/Stem Cells and Their Subsets by a High Throughput Technology [J].

Baer, Patrick C. ;

Kuci, Selim ;

Krause, Michael ;

Kuci, Zyrafete ;

Zielen, Stefan ;

Geiger, Helmut ;

Bader, Peter ;

Schubert, Ralf .

STEM CELLS AND DEVELOPMENT, 2013, 22 (02) :330-339

[3] Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT) [J].

Bourin, Philippe ;

Bunnell, Bruce A. ;

Casteilla, Louis ;

Dominici, Massimo ;

Katz, Adam J. ;

March, Keith L. ;

Redl, Heinz ;

Rubin, J. Peter ;

Yoshimura, Kotaro ;

Gimble, Jeffrey M. .

CYTOTHERAPY, 2013, 15 (06) :641-648

[4] Abdominal obesity and metabolic syndrome [J].

Despres, Jean-Pierre ;

Lemieux, Isabelle .

NATURE, 2006, 444 (7121) :881-887

[5] Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].

Dominici, M. ;

Le Blanc, K. ;

Mueller, I. ;

Slaper-Cortenbach, I. ;

Marini, F. C. ;

Krause, D. S. ;

Deans, R. J. ;

Keating, A. ;

Prockop, D. J. ;

Horwitz, E. M. .

CYTOTHERAPY, 2006, 8 (04) :315-317

[6] Fat tissue: an underappreciated source of stem cells for biotechnology [J].

Fraser, JK ;

Wulur, I ;

Alfonso, Z ;

Hedrick, MH .

TRENDS IN BIOTECHNOLOGY, 2006, 24 (04) :150-154

[7] CD200 is a ligand for all members of the CD200R family of immunoregulatory molecules [J].

Gorczynski, R ;

Chen, ZQ ;

Kai, Y ;

Lee, L ;

Wong, S ;

Marsden, PA .

JOURNAL OF IMMUNOLOGY, 2004, 172 (12) :7744-7749

[8] Surface protein characterization of human adipose tissue-derived stromal cells [J].

Gronthos, S ;

Franklin, DM ;

Leddy, HA ;

Robey, PG ;

Storms, RW ;

Gimble, JM .

JOURNAL OF CELLULAR PHYSIOLOGY, 2001, 189 (01) :54-63

[9] Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells [J].

Katz, AJ ;

Tholpady, A ;

Tholpady, SS ;

Shang, HL ;

Ogle, RC .

STEM CELLS, 2005, 23 (03) :412-423

[10] Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells [J].

Kelly, Olivia G. ;

Chan, Man Yin ;

Martinson, Laura A. ;

Kadoya, Kuniko ;

Ostertag, Traci M. ;

Ross, Kelly G. ;

Richardson, Mike ;

Carpenter, Melissa K. ;

D'Amour, Kevin A. ;

Kroon, Evert ;

Moorman, Mark ;

Baetge, Emmanuel E. ;

Bang, Anne G. .

NATURE BIOTECHNOLOGY, 2011, 29 (08) :750-U114

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