Efficacy of ozone on survival and permeability of oral microorganisms

被引:148
作者
Nagayoshi, M
Fukuizumi, T
Kitamura, C
Yano, J
Terashita, M
Nishihara, T
机构
[1] Kyushu Dent Coll, Dept Oral Microbiol, Kokurakita Ku, Kitakyushu, Fukuoka 8038580, Japan
[2] Kyushu Dent Coll, Dept Operat Dent & Endodont, Kitakyushu, Fukuoka 8038580, Japan
来源
ORAL MICROBIOLOGY AND IMMUNOLOGY | 2004年 / 19卷 / 04期
关键词
antimicrobial activity; dental plaque biofilm; disinfectant; oral microorganisms; ozone;
D O I
10.1111/j.1399-302X.2004.00146.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
In the present study, we examined the effect of ozonated water on oral microorganisms and dental plaque. Almost no microorganisms were detected after being treated with ozonated water (4 mg/l) for 10 s. To estimate the ozonated water-treated Streptococcus mutans, bacterial cells were stained with LIVE/DEAD((R))BacLight(TM) Bacterial Viability Kit. Fluorescence microscopic analysis revealed that S. mutans cells were killed instantaneously in ozonated water. Some breakage of ozonated water-treated S. mutans was found by electron microscopy. When the experimental dental plaque was exposed to ozonated water, the number of viable S. mutans remarkably decreased. Ozonated water strongly inhibited the accumulation of experimental dental plaque in vitro. After the dental plaque samples from human subjects were exposed to ozonated water in vitro, almost no viable bacterial cells were detected. These results suggest that ozonated water should be useful in reducing the infections caused by oral microorganisms in dental plaque.
引用
收藏
页码:240 / 246
页数:7
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