Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli

被引:21
作者
Dawson, S. E. [1 ]
Gibreel, T. [1 ]
Nicolaou, N. [2 ,3 ]
AlRabiah, H. [2 ,3 ]
Xu, Y. [2 ,3 ]
Goodacre, R. [2 ,3 ]
Upton, M. [1 ,4 ,5 ]
机构
[1] Univ Manchester, Sch Med, Microbiol & Virol Unit, Manchester M13 9PL, Lancs, England
[2] Univ Manchester, Sch Chem, Manchester M13 9PL, Lancs, England
[3] Univ Manchester, Manchester Inst Biotechnol, Manchester M13 9PL, Lancs, England
[4] Univ Plymouth, Sch Biomed & Healthcare Sci, Plymouth PL4 8AA, Devon, England
[5] Univ Manchester, Sch Med, Manchester M13 9PL, Lancs, England
关键词
URINARY-TRACT-INFECTIONS; CLONAL GROUPS; DISCRIMINATION; IDENTIFICATION; BACTERIA; NORTHWEST;
D O I
10.1007/s10096-013-2036-0
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19 % for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.
引用
收藏
页码:983 / 988
页数:6
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