Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli

被引：21

作者：

Dawson, S. E. ^{[1
]}

Gibreel, T. ^{[1
]}

Nicolaou, N. ^{[2
,3
]}

AlRabiah, H. ^{[2
,3
]}

Xu, Y. ^{[2
,3
]}

Goodacre, R. ^{[2
,3
]}

Upton, M. ^{[1
,4
,5
]}

机构：

[1] Univ Manchester, Sch Med, Microbiol & Virol Unit, Manchester M13 9PL, Lancs, England

[2] Univ Manchester, Sch Chem, Manchester M13 9PL, Lancs, England

[3] Univ Manchester, Manchester Inst Biotechnol, Manchester M13 9PL, Lancs, England

[4] Univ Plymouth, Sch Biomed & Healthcare Sci, Plymouth PL4 8AA, Devon, England

[5] Univ Manchester, Sch Med, Manchester M13 9PL, Lancs, England

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2014年 / 33卷 / 06期

关键词：

URINARY-TRACT-INFECTIONS; CLONAL GROUPS; DISCRIMINATION; IDENTIFICATION; BACTERIA; NORTHWEST;

D O I：

10.1007/s10096-013-2036-0

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19 % for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.

引用

页码：983 / 988

页数：6

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