Base extrusion is found at helical junctions between right- and left-handed forms of DNA and RNA

被引：26

作者：

Kim, Doyoun ^{[1
]}

Reddy, Sanjith ^{[1
]}

Kim, Dong Young ^{[1
]}

Rich, Alexander ^{[2
]}

Lee, Sangho ^{[3
]}

Kim, Kyeong Kyu ^{[1
]}

Kim, Yang-Gyun ^{[4
]}

机构：

[1] Sungkyunkwan Univ, Sch Med, Samsung Biomed Res Inst, Dept Mol Cell Biol, Suwon 440746, South Korea

[2] MIT, Dept Biol, Cambridge, MA 02139 USA

[3] Sungkyunkwan Univ, Dept Biol Sci, Suwon 440746, South Korea

[4] Sungkyunkwan Univ, Dept Chem, Suwon 440746, South Korea

来源：

NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 13期

关键词：

Z-ALPHA DOMAIN; EDITING ENZYME ADAR1; CRYSTAL-STRUCTURE; B-DNA; HOLLIDAY JUNCTION; STRUCTURAL BASIS; 2-AMINOPURINE; FLUORESCENCE; BINDING; METHYLTRANSFERASE;

D O I：

10.1093/nar/gkp364

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Base extrusion is a major structural feature at the junction between B- and Z-DNA (the B-Z junction) where a base pair is broken, and the two bases are extruded from the double helix. Despite the demonstration of base extrusion at the B-Z junction, it is not clear whether a similar base extrusion occurs at other types of junctions involving the left-handed Z conformation. Here, we investigate structural changes of bases at three Z-form junctions: DNA B-Z and Z-Z and RNA A-Z junctions. By monitoring fluorescently labeled duplex nucleic acids using 2-aminopurines at various positions relative to the junction point, we show that base extrusion occurs not only at the DNA B-Z junction, but also at the RNA A-Z and DNA Z-Z junctions. Our data suggest that base extrusion is a general feature of Z-form nucleic-acid junctions.

引用

页码：4353 / 4359

页数：7

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