Protective role for plasmid DNA-mediated VIP gene transfer in non-obese diabetic mice

被引：26
作者：
Herrera, Juan Luis
Fernandez-Montesinos, Rafael
Gonzalez-Rey, Elena
Delgado, Mario
Pozo, David
机构：
[1] Univ Seville, Sch Med, Dept Med Biochem & Mol Biol, E-41009 Seville, Spain
[2] CSIC, Inst Biomed & Parasitol, Granada, Spain
来源：
VIP, PACAP, AND RELATED PEPTIDES: FROM GENE TO THERAPY | 2006年 / 1070卷
关键词：
vasoactive intestinal peptide; gene delivery; diabetes; neuroimmunology;
D O I：
10.1196/annals.1317.041
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Studies focused on the development of diabetes in NOD mice-a model for human type 1 diabetes-have revealed that an autoimmune inflammatory process is produced by the effect of Th1 cells and. their secreted cytokines. DNA vaccination has been shown to be an effective method for modulating immunity in viral infections and experimental autoimmune diseases, including diabetes. VIP's immunomodulatory properties are partly mediated by skewing the pattern of cytokines from a proinflammatory response to an anti-inflammatory response. Using gene delivery to express VIP, we interfered in the immune process leading to diabetes in prone, cyclophosphamide-treated NOD mice. Our results extend the role of VIP in the control of immunoregulatory networks and open new perspectives for immunointervention through VIP-based gene therapy.
引用
收藏
页码：337 / 341
页数：5
相关论文
共 12 条
  • [1] The NOD mouse model of type 1 diabetes: As good as it gets?
    Atkinson, MA
    Leiter, EH
    [J]. NATURE MEDICINE, 1999, 5 (06) : 601 - 604
  • [2] A role for innate immunity in type 1 diabetes?
    Beyan, H
    Buckley, LR
    Yousaf, N
    Londei, M
    Leslie, R
    [J]. DIABETES-METABOLISM RESEARCH AND REVIEWS, 2003, 19 (02) : 89 - 100
  • [3] Vasoactive intestinal peptide in the immune system: potential therapeutic role in inflammatory and autoimmune diseases
    Delgado, M
    Abad, C
    Martinez, C
    Juarranz, MG
    Arranz, A
    Gomariz, RP
    Leceta, J
    [J]. JOURNAL OF MOLECULAR MEDICINE-JMM, 2002, 80 (01): : 16 - 24
  • [4] The significance of vasoactive intestinal peptide in immunomodulation
    Delgado, M
    Pozo, D
    Ganea, D
    [J]. PHARMACOLOGICAL REVIEWS, 2004, 56 (02) : 249 - 290
  • [5] Driven to autoimmunity: The NOD mouse
    Gallegos, AM
    Bevan, MJ
    [J]. CELL, 2004, 117 (02) : 149 - 151
  • [6] Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) as modulators of both innate and adaptive immunity
    Ganea, D
    Delgado, M
    [J]. CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 2002, 13 (03) : 229 - 237
  • [7] DEMONSTRATION OF IMMUNOREACTIVE VASOACTIVE-INTESTINAL-PEPTIDE (IR-VIP) AND SOMATOSTATIN (IR-SOM) IN RAT THYMUS
    GOMARIZ, RP
    LORENZO, MJ
    CACICEDO, L
    VICENTE, A
    ZAPATA, AG
    [J]. BRAIN BEHAVIOR AND IMMUNITY, 1990, 4 (02) : 151 - 161
  • [8] INTERACTION OF VASOACTIVE INTESTINAL PEPTIDE WITH HUMAN-BLOOD MONONUCLEAR-CELLS
    GUERRERO, JM
    PRIETO, JC
    ELORZA, FL
    RAMIREZ, R
    GOBERNA, R
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1981, 21 (02) : 151 - 160
  • [9] Kukreja A, 2002, J CLIN INVEST, V109, P131, DOI 10.1172/JCI13605
  • [10] ODORISIO MS, 1981, J IMMUNOL, V127, P2551
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