Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species

被引：51
作者：
Massonet, C
Van Eldere, J
Vaneechoutte, M
De Baere, T
Verhaegen, J
Lagrou, K
机构：
[1] Univ Hosp Gasthuisberg, Rega Inst, Dept Immunol & Microbiol, B-3000 Louvain, Belgium
[2] Univ Hosp Gasthuisberg, Lab Geneeskunde, B-3000 Louvain, Belgium
[3] Ghent Univ Hosp, Dept Clin Microbiol & Immunol, B-9000 Ghent, Belgium
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 05期
关键词：
D O I：
10.1128/JCM.42.5.2209-2211.2004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A total of 61 clinical yeast isolates of Candida, Cryptococcus, Blastoschizomyces, and Saccharomyces spp. were used to compare two identification techniques, VITEK 2 and ITS2-fragment length polymorphism analysis (ITS2-FLP), with ID32C as the reference method. ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-freezing DNA extraction identified 55 isolates. VITEK 2 identified 41 isolates correctly.
引用
收藏
页码：2209 / 2211
页数：3
相关论文
共 17 条
[1]   Evaluation of six commercial systems for identification of medically important yeasts [J].
Buchaille L. ;
Freydière A.M. ;
Guinet R. ;
Gille Y. .
European Journal of Clinical Microbiology and Infectious Diseases, 1998, 17 (7) :479-488
[2]  
Chen YC, 2000, J CLIN MICROBIOL, V38, P2302
[3]   Evaluation of the microbial identification system for identification of clinically isolated yeasts [J].
Crist, AE ;
Johnson, LM ;
Burke, PJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (10) :2408-2410
[4]   Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region [J].
De Baere, Thierry ;
Claeys, Geert ;
Swinne, Danielle ;
Massonet, Caroline ;
Verschraegen, Gerda ;
Muylaert, An ;
Vaneechoutte, Mario .
BMC MICROBIOLOGY, 2002, 2 (1) :1-8
[5]   Comparison of four methodologies for rapid and cost-effective identification of Candida glabrata [J].
Fenn, JP ;
Billetdeaux, E ;
Segal, H ;
Skodack-Jones, L ;
Padilla, PE ;
Bale, M ;
Carroll, K .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) :3387-3389
[6]   Identification of Candida glabrata by a 30-second trehalase test [J].
Freydière, AM ;
Parant, F ;
Noel-Baron, F ;
Crepy, M ;
Treny, A ;
Raberin, H ;
Davidson, A ;
Odds, FC .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (10) :3602-3605
[7]   Comparison of the new API Candida system to the ID 32C system for identification of clinically important yeast species [J].
FrickerHidalgo, H ;
Vandapel, O ;
Duchesne, MA ;
Mazoyer, MA ;
Monget, D ;
Lardy, B ;
Lebeau, B ;
Freney, J ;
AmbroiseThomas, P ;
Grillot, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (07) :1846-1848
[8]  
FRICKERHIDALGO H, 1995, ANN BIOL CLIN-PARIS, V53, P221
[9]   Evaluation of the VITEK 2 system for rapid identification of yeasts and yeast-like organisms [J].
Graf, B ;
Adam, T ;
Zill, E ;
Göbel, UB .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1782-1785
[10]  
GUTIERREZ J, 1994, ANN BIOL CLIN-PARIS, V52, P443
12