Detection of Turkey, Duck, and Guinea Fowl Egg in Hen Egg Products by Species-Specific PCR

被引：6

作者：

Nau, Francoise ^{[1
,2
]}

Desert, Colette ^{[3
]}

Cochet, Marie-Francoise ^{[2
]}

Pasco, Maryvonne ^{[2
]}

Jan, Sophie ^{[2
]}

Baron, Florence ^{[2
]}

Lagarrigue, Sandrine ^{[3
]}

Guerin-Dubiard, Catherine ^{[2
]}

机构：

[1] AGROCAMPUS OUEST, INRA, UMR1253, F-35042 Rennes, France

[2] AGROCAMPUS OUEST, INRA, Sci & Technol Lait & OEuf UMR1253, F-35000 Rennes, France

[3] AGROCAMPUS OUEST, INRA, Genet Anim UMR598, F-35000 Rennes, France

来源：

FOOD ANALYTICAL METHODS | 2009年 / 2卷 / 03期

关键词：

Egg Product; Hen; Duck; Turkey; Guinea Fowl; Species-Specific PCR; POLYMERASE-CHAIN-REACTION; FRAGMENT LENGTH POLYMORPHISM; MITOCHONDRIAL-DNA; FOOD-PRODUCTS; IDENTIFICATION; TIME; AUTHENTICATION; AMPLIFICATION; SAMPLES; CHEESE;

D O I：

10.1007/s12161-009-9077-0

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A simplex polymerase chain reaction (PCR) has been applied for the specific detection of hen, duck, turkey, and guinea fowl in egg products using species-specific primers targeting the mitochondrial cytochrome b genes. The species-specific PCR yielded excellent results for identification of duck, turkey, and guinea fowl eggs in hen egg products, since the detection of 0.1% of each species was achieved, in liquid egg products as well as in powders. The proposed method is then a potentially reliable and suitable technique in routine food analysis for the research of fraudulent species mixture practices.

引用

页码：231 / 238

页数：8

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