Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion

被引：35

作者：

da Silva, Claudio V. ^{[1
]}

Kawashita, Silvia Y. ^{[1
]}

Probst, Christian M. ^{[2
,3
]}

Dallagiovanna, Bruno ^{[2
,3
]}

Cruz, Mario C. ^{[1
]}

da Silva, Erika A. ^{[1
]}

Souto-Padron, Thais C. B. S. ^{[4
]}

Krieger, Marco A. ^{[2
,3
]}

Goldenberg, Samuel ^{[2
,3
]}

Briones, Marcelo R. S. ^{[1
]}

Andrews, Norma W. ^{[5
]}

Mortara, Renato A. ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil

[2] Fiocruz MS, Inst Biol Mol Parana, Curitiba, Parana, Brazil

[3] Fiocruz MS, ICC, Curitiba, Parana, Brazil

[4] Univ Fed Rio de Janeiro, Dept Microbiol, BR-21941 Rio De Janeiro, Brazil

[5] Yale Univ, Sch Med, Sect Microbial Pathogenesis, New Haven, CT USA

来源：

MICROBES AND INFECTION | 2009年 / 11卷 / 05期

基金：

巴西圣保罗研究基金会;

关键词：

Trypanosoma cruzi; Cell invasion; Amastigote; Metacyclic trypomastigote; MAJOR PHYLOGENETIC LINEAGES; METACYCLIC TRYPOMASTIGOTES; MONOCLONAL-ANTIBODIES; SURFACE-MOLECULE; EPITHELIAL-CELLS; INFECTIVE FORMS; AMASTIGOTES; GLYCOPROTEIN; INVOLVEMENT; EXPRESSION;

D O I：

10.1016/j.micinf.2009.03.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence that codes for a 21 kDa protein that showed high probability of being secreted was selected. After cloning this protein sequence, the results showed that it was a ubiquitous protein and secreted by extracellular amastigotes. The recombinant form (P21-His(6)) adhered to HeLa cells in a dose-dependent manner. Pretreatment of host cells with P21-His(6) inhibited cell invasion by extracellular amastigotes from G and CL strains. On the other hand, when the protein was added to host cells at the same time as amastigotes, an increase in cell invasion was observed. Host-cell pretreatment with P21-His(6) augmented invasion by metacyclic trypomastigotes. Moreover, polyclonal antibody anti-P21 inhibited invasion only by extracellular amastigotes and metacyclic trypomastigotes from G strain. These results suggested that P21 might be involved in T. cruzi cell invasion. We hypothesize that P21 could be secreted in the juxtaposition parasite-host cell and triggers signaling events yet unknown that lead to parasite internalization. (C) 2009 Elsevier Masson SAS. All rights reserved.

引用

页码：563 / 570

页数：8

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