Biosurfactant-enhanced bioremediation of aged polycyclic aromatic hydrocarbons (PAHs) in creosote contaminated soil

被引：147

作者：

Bezza, Fisseha Andualem ^{[1
]}

Chirwa, Evans M. Nkhalambayausi ^{[1
]}

机构：

[1] Univ Pretoria, Dept Chem Engn, Water Utilisat & Environm Engn Div, ZA-0002 Pretoria, South Africa

来源：

CHEMOSPHERE | 2016年 / 144卷

基金：

新加坡国家研究基金会;

关键词：

Biosurfactant; Creosote degradation; In situ biosurfactant production; Bio-slurry reactor; BIODEGRADATION KINETICS; CRUDE-OIL; STRAIN; PYRENE; SOLUBILIZATION; PHENANTHRENE; DEGRADATION; BACTERIUM; MIXTURES; SYSTEMS;

D O I：

10.1016/j.chemosphere.2015.08.027

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The potential for biological treatment of an environment contaminated by complex petrochemical contaminants was evaluated using creosote contaminated soil in ex situ bio-slurry reactors. The efficacy of biosurfactant application and stimulation of in situ biosurfactant production was investigated. The biosurfactant produced was purified and characterised using Fourier transform infrared (FTIR) spectroscopy. Biosurfactant enhanced degradation of PAHs was 86.5% (with addition of biosurfactant) and 57% in controls with no biosurfactant and nutrient amendments after incubation for 45 days. A slight decrease in degradation rate observed in the simultaneous biosurfactant and nutrient, NH4NO3 and KH2PO4, supplemented microcosm can be attributed to preferential microbial consumption of the biosurfactant supplemented. The overall removal of PAHs was determined to be mass transport limited since the dissolution rate caused by the biosurfactant enhanced the bioavailability of the PAHs to the microorganisms. The consortium culture was predominated by the aromatic ring-cleaving species Bacillus stratosphericus, Bacillus subtilis, Bacillus megaterium, and Pseudomonas aeruginosa. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：635 / 644

页数：10

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