Essential Oils as Alternative Biocides for the Preservation of Waterlogged Archaeological Wood

被引：41

作者：

Antonelli, Federica ^{[1
]}

Bartolini, Marco ^{[2
]}

Plissonnier, Marie-Laure ^{[3
]}

Esposito, Alfonso ^{[4
]}

Galotta, Giulia ^{[2
]}

Ricci, Sandra ^{[2
]}

Petriaggi, Barbara Davidde ^{[5
]}

Pedone, Cristian ^{[6
]}

Di Giovanni, Antonella ^{[6
]}

Piazza, Silvano ^{[4
,7
]}

Guerrieri, Francesca ^{[3
]}

Romagnoli, Manuela ^{[1
]}

机构：

[1] Tuscia Univ, Dept Innovat Biol, Agrofood & Forestry Syst DIBAF, I-01100 Viterbo, Italy

[2] Minist Cultural Heritage & Act & Tourism MIBACT, Ist Cent Restauro ICR, Biol Lab, I-00153 Rome, Italy

[3] Canc Res Ctr Lyon CRCL, U1052, Epigenet & Epigen Hepatocellular Carcinoma, F-69424 Lyon 03, France

[4] Univ Trento, Dept Cellular Computat & Integrat Biol CIBIO, I-38123 Trento, Italy

[5] Minist Cultural Heritage & Act & Tourism MIBACT, Ist Cent Restauro ICR, Underwater Archaeol Operat Unit, I-00153 Rome, Italy

[6] Minist Cultural Heritage & Act & Tourism MIBACT, Ist Cent Restauro ICR, Restorat Lab Organ Excavat Mat, I-00153 Rome, Italy

[7] Int Ctr Genet Engn & Biotechnol, Computat Biol, I-34149 Trieste, Italy

来源：

MICROORGANISMS | 2020年 / 8卷 / 12期

关键词：

cinnamon; wild thyme; common thyme; cultural analyses; ATP bioluminescence; NGS; minimum inhibitory concentration (MIC); WAW; archaeological wood biodegradation; archaeological wood restoration; ANTIFUNGAL ACTIVITY; ATP BIOLUMINESCENCE; CARVACROL; EUGENOL; CINNAMALDEHYDE; CONSERVATION; FOOD; MOLD; CONTAMINATION; MONOTERPENES;

D O I：

10.3390/microorganisms8122015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Waterlogged archaeological wood is exposed to a high risk of biological degradation during the post-excavation phases of storage and restoration. For this reason, often biocides must be used to preserve wooden remains. In the present work three essential oils (cinnamon, wild thyme, and common thyme) were tested as possible alternative biocides to use in the preservation of waterlogged archaeological wood. The oils were first tested in vitro to establish the minimum inhibitory concentration (MIC) and to evaluate the biocidal activity on selected fungal strains. Then, the established MIC was applied on waterlogged archaeological wood samples and during an actual restoration treatment. The effectiveness of the oils was evaluated through cultural analyses, ATP quantification, and next-generation sequencing. The results showed that the oils caused a significant decrease in the vitality of fungal mycelia grown in vitro and of the microbiota present in treated wood and storage water. Furthermore, an influence on the composition of the bacterial communities of treated wood samples was observed. Although further tests are needed to evaluate interferences with the materials used during restoration procedures, essential oils could be considered as a possible alternative to the currently used biocide.

引用

页码：1 / 25

页数：25

共 106 条

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