HIV-1 mRNA 3′ End Processing Is Distinctively Regulated by eIF3f, CDK11, and Splice Factor 9G8

被引：42
作者：
Valente, Susana T. [1 ,3 ]
Gilmartin, Greg M. [2 ]
Venkatarama, Krishnan [2 ]
Arriagada, Gloria [1 ,3 ]
Goff, Stephen P. [1 ,3 ]
机构：
[1] Columbia Univ, Dept Biochem & Mol Biophys, Coll Phys & Surg, New York, NY 10032 USA
[2] Univ Vermont, Dept Microbiol & Mol Genet, Markey Ctr Mol Genet, Vermont Canc Ctr, Burlington, VT 05405 USA
[3] Columbia Univ, Howard Hughes Med Inst, Coll Phys & Surg, New York, NY 10032 USA
来源：
MOLECULAR CELL | 2009年 / 36卷 / 02期
关键词：
CLEAVAGE FACTOR-I; UPSTREAM SEQUENCE ELEMENT; PROTEIN-PROTEIN; GENE-EXPRESSION; POLY(A) SIGNAL; SR PROTEINS; U1; SNRNP; POLYADENYLATION; APOPTOSIS; CYCLIN;
D O I：
10.1016/j.molcel.2009.10.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A genetic screen previously identified the N-terminal 91 amino acids of the eukaryotic initiation factor 3 subunit f (N91-eIF3f) as a potent inhibitor of HIV-1 replication. Overexpression of N91-eIF3f or full-length eIF3f reduced the level of HIV-1 mRNAs in the infected cell. Here we show that N91-eIF3f and eIF3f act by specifically blocking the 3' end processing of the HIV-1 pre-mRNA both in vivo and in vitro. Furthermore, the results suggest that eIF3f mediates this restriction of HIV-1 expression through the previously unsuspected involvement of a set of factors that includes eIF3f, the SR protein 9G8, and the cyclin-dependent kinase 11 (CDK11). eIF3f affects HIV-1 3' end processing by modulating the sequence-specific recognition of the HIV-1 pre-mRNA by 9G8.
引用
收藏
页码：279 / 289
页数：11
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