RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report

被引:3
作者
Banerjee, Samiddha [1 ]
Pal, Aruna [2 ]
Pal, Abantika [3 ]
Mandal, Subhas Chandra [2 ]
Chatterjee, Paresh Nath [2 ]
Chatterjee, Jayanta Kumar [1 ]
机构
[1] Visva Bharati Univ, Dept Anim Sci, Bolpur, India
[2] West Bengal Univ Anim & Fishery Sci, Dept LFC, Kolkata, India
[3] Indian Inst Technol, Dept Comp Sci, Kharagpur, W Bengal, India
关键词
RIG-I; innate immunity; parasite; Haemonchus contortus; sheep; RECOGNITION; EXPRESSION; RESISTANCE; RECEPTORS; CD14; WEB;
D O I
10.3389/fimmu.2020.534705
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Retinoic acid inducible gene I (RIG-I) is associated to the DExD/H box RNA helicases. It is a pattern recognition receptor (PRR), playing a crucial role in the system and is a germ line encoded host sensor to perceive pathogen-associated molecular patterns (PAMPs). So far, reports are available for the role of RIG-I in antiviral immunity. This is the first report in which we have documented the role of RIG-I in parasitic immunity. Haemonchus contortus is a deadly parasite affecting the sheep industry, which has a tremendous economic importance, and the parasite is reported to be prevalent in the hot and humid agroclimatic region. We characterize the RIG-I gene in sheep (Ovis aries) and identify the important domains or binding sites with Haemonchus contortus through in silico studies. Differential mRNA expression analysis reveals upregulation of the RIG-I gene in the abomasum of infected sheep compared with that of healthy sheep, further confirming the findings. Thus, it is evident that, in infected sheep, expression of RIG-I is triggered for binding to more pathogens (Haemonchus contortus). Genetically similar studies with humans and other livestock species were conducted to reveal that sheep may be efficiently using a model organism for studying the role of RIG-I in antiparasitic immunity in humans.
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