Reduced graphene oxide and gold nanoparticle composite-based solid-phase extraction coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry for the determination of 9 mycotoxins in milk

被引：69

作者：

Jiang, Keqiu ^{[1
]}

Huang, Qingwen ^{[1
]}

Fan, Kai ^{[2
]}

Wu, Lidong ^{[3
]}

Nie, Dongxia ^{[2
]}

Guo, Wenbo ^{[2
]}

Wu, Yongjiang ^{[1
]}

Han, Zheng ^{[2
]}

机构：

[1] Zhejiang Univ, Coll Pharmaceut Sci, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China

[2] Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Shanghai Key Lab Protected Hort Technol,Minist Ag, Lab Qual & Safety Risk Assessment Agroprod Shangh, Shanghai 201403, Peoples R China

[3] Chinese Acad Fishery Sci, Minist Agr, Key Lab Control Qual & Safety Aquat Prod, Beijing 100141, Peoples R China

来源：

FOOD CHEMISTRY | 2018年 / 264卷

基金：

中国国家自然科学基金;

关键词：

Milk; Reduced graphene oxide and gold nanoparticle composite; Mycotoxins; Solid-phase extraction; Ultra-high-performance liquid chromatography-tandem mass spectrometry; AFLATOXIN M-1; DAIRY-PRODUCTS; COW MILK; QUANTIFICATION; ZEARALENONE; MS/MS; TRICHOTHECENES; FLUORESCENCE; OCHRATOXIN; VALIDATION;

D O I：

10.1016/j.foodchem.2018.05.041

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A reliable solid-phase extraction (SPE) procedure using reduced graphene oxide and gold nanoparticle (rGO/Au) composite as sorbent was proposed for purification and enrichment of aflatoxin B-1 (AFB(1)), aflatoxin M-1 (AFM(1)), ochratoxin A (OTA), zearalenone (ZEA), alpha-zearalenol (alpha-ZOL), beta-zearalenol (beta-ZOL), zearalanone (ZAN), alpha-zearalanol (alpha-ZAL) and beta-zearalanol (beta-ZAL) in milk. Main parameters affecting the performance of SPE procedure were thoroughly investigated. The optimized conditions included 2% acetonitrile in water as the loading solution, 5% methanol in water as the washing solution, and methanol/acetonitrile/formic acid (50/49/1, v/v/v) as the elution solvent. Satisfactory linearity (R-2 >= 0.992), high sensitivity (limit of quantification in the range of 0.02-0.18 ng mL(-1)), adequate recovery (70.2-111.2%), and acceptable precision (RSD, 2.0-14.9%) were obtained when the optimal sample pretreatment protocol was combined with ultra-high-performance liquid chromatography-tandem mass spectrometry detection. The applicability of the validated method was further verified in real milk samples.

引用

页码：218 / 225

页数：8

共 40 条

[1] Comparison of the efficiency of different extraction methods for the simultaneous determination of mycotoxins and pesticides in milk samples by ultra high-performance liquid chromatography-tandem mass spectrometry [J].