Model Lipid Membranes on a Tunable Polymer Cushion

被引：46

作者：

Smith, Hillary L. ^{[1
]}

Jablin, Michael S. ^{[1
]}

Vidyasagar, Ajay ^{[2
]}

Saiz, Jessica ^{[1
]}

Watkins, Erik ^{[1
,3
]}

Toomey, Ryan ^{[2
]}

Hurd, Alan J. ^{[1
]}

Majewski, Jaroslaw ^{[1
]}

机构：

[1] Los Alamos Natl Lab, Manuel Lujan Jr Neutron Scattering Ctr, Los Alamos, NM 87545 USA

[2] Univ S Florida, Dept Chem & Biomed Engn, Tampa, FL 33620 USA

[3] Univ Calif Davis, Biophys Grad Grp, Davis, CA 95616 USA

来源：

PHYSICAL REVIEW LETTERS | 2009年 / 102卷 / 22期

基金：

美国国家科学基金会;

关键词：

NEUTRON-SCATTERING; DYNAMICS; SURFACES; GEL;

D O I：

10.1103/PhysRevLett.102.228102

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

A hydrated, surface-tethered polymer network capable of fivefold change in thickness over a 25-37 degrees C temperature range has been demonstrated via neutron reflectivity and fluorescence microscopy to be a novel support for single lipid bilayers in a liquid environment. As the polymer swells from 170 to 900 A, it promotes both in- and out-of-plane fluctuations of the supported membrane. The cushioned bilayer proved to be very robust, remaining structurally intact for 16 days and many temperature cycles. The promotion of membrane fluctuations offers far-reaching applications for this system as a surrogate biomembrane.

引用

页数：4

共 15 条

[1] Investigating the interface of superhydrophobic surfaces in contact with water
Doshi, DA
Shah, PB
Singh, S
Branson, ED
Malanoski, AP
Watkins, EB
Majewski, J
van Swol, F
Brinker, CJ
[J]. LANGMUIR, 2005, 21 (17) : 7805 - 7811
[2] Dynamics of swollen gel layers anchored to solid surfaces
Gianneli, Maria
Roskamp, Robert F.
Jonas, Ulrich
Loppinet, Benoit
Fytas, George
Knoll, Wolfgang
[J]. SOFT MATTER, 2008, 4 (07) : 1443 - 1447
[3] Bending mechanics and molecular organization in biological membranes
Groves, Jay T.
[J]. ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 2007, 58 : 697 - 717
[4] Molecular-scale structural and functional characterization of sparsely tethered bilayer lipid membranes
McGillivray, Duncan J.
Valincius, Gintaras
Vanderah, David J.
Febo-Ayala, Wilma
Woodward, John T.
Heinrich, Frank
Kasianowicz, John J.
Losche, Mathias
[J]. BIOINTERPHASES, 2007, 2 (01) : 21 - 33
[5] Co-refinement of multiple-contrast neutron/X-ray reflectivity data using MOTOFIT
Nelson, A
[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2006, 39 : 273 - 276
[6] NIELSEN JA, 1986, PHYSICA A, V140, P376
[7] SURFACE STUDIES OF SOLIDS BY TOTAL REFLECTION OF X-RAYS
PARRATT, LG
[J]. PHYSICAL REVIEW, 1954, 95 (02): : 359 - 369
[8] Stress and fold localization in thin elastic membranes
Pocivavsek, Luka
Dellsy, Robert
Kern, Andrew
Johnson, Sebastian
Lin, Binhua
Lee, Ka Yee C.
Cerda, Enrique
[J]. SCIENCE, 2008, 320 (5878) : 912 - 916
[9] Supported membranes: Scientific and practical applications
Sackmann, E
[J]. SCIENCE, 1996, 271 (5245) : 43 - 48
[10] SURFACE-PATTERN EVOLUTION IN A SWELLING GEL UNDER A GEOMETRICAL CONSTRAINT - DIRECT OBSERVATION OF FOLD STRUCTURE AND ITS COARSENING DYNAMICS
TANAKA, H
SIGEHUZI, T
[J]. PHYSICAL REVIEW E, 1994, 49 (01): : R39 - R42

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