Enhanced expression of neuronal nitric oxide synthase and phospholipase C-γ1 in regenerating murine neuronal cells by pulsed electromagnetic field

被引：37
作者：
Kim, SS
Shin, HJ
Eom, DW
Huh, JR
Woo, Y
Kim, H
Ryu, SH
Suh, PG
Kim, MJ
Kim, JY
Koo, TW
Cho, YH
Chung, SM
机构：
[1] Univ Ulsan, Coll Med, Dept Pathol, Ulsan 6827060, South Korea
[2] Ewha Womans Univ, Coll Med, Dept Otolaryngol, Seoul 158710, South Korea
[3] Univ Ulsan, Coll Med, Dept Neurol, Ulsan 682060, South Korea
[4] Pohang Coll Sci & Technol, Dept Life Sci, Pohang 790784, South Korea
来源：
EXPERIMENTAL AND MOLECULAR MEDICINE | 2002年 / 34卷 / 01期
关键词：
PEMF; PLC-gamma; 1; NOS; S-100;
D O I：
10.1038/emm.2002.8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pulsed electromagnetic field (PEMF) has been shown to improve the rate of peripheral nerve regeneration. In the present study we investigated the expression of neuronal nitric oxide synthase (nNOS) and phospholipase C-gamma1 (PLC-gamma1) in regenerating rat laryngeal nerves during the exposure to PEMF after surgical transection and reanastomosis. Axons were found to regenerate into the distal stump nearly twice faster in PEMF-exposed animals than in the control. Consistently, motor function was better recovered in PEMF-treated rats. The expression of nNOS and PLC-gamma1 was highly enhanced in the regenerated nerves.
引用
收藏
页码：53 / 59
页数：7
相关论文
共 20 条
[1]   Electromagnetic field induced changes in lipid second messengers [J].
Clejan, S ;
Ide, C ;
Walker, C ;
Wolf, E ;
Corb, M ;
Beckman, B .
JOURNAL OF LIPID MEDIATORS AND CELL SIGNALLING, 1996, 13 (03) :301-324
[2]   EFFECT OF A HIGH-INTENSITY STATIC MAGNETIC-FIELD ON SCIATIC-NERVE REGENERATION IN THE RAT [J].
CORDEIRO, PG ;
SECKEL, BR ;
MILLER, CD ;
GROSS, PT ;
WISE, RE .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1989, 83 (02) :301-306
[3]  
DIBIRDIK I, 1988, J BIOL CHEM, V13, P4035
[4]   Exogenous phospholipase C stimulates epithelial cell migration and integrin expression in vitro [J].
Firth, JD ;
Putnins, EE ;
Larjava, H ;
Uitto, VJ .
WOUND REPAIR AND REGENERATION, 2001, 9 (02) :86-94
[5]  
GONSALEZ HT, 1999, J NEUROSCI RES, V15, P98
[6]  
GONSALEZ MF, 1989, BRAIN RESES B, V18, P417
[7]  
ILKER D, 1998, J BIOL CHEM, V13, P4035
[8]  
JANET LW, 1994, EXP NEUROL, V125, P302
[9]  
JOHN MB, 1998, ARCH OTOLARYNGOL, V124, P383
[10]   Immunohistochemical localization of eight phospholipase C isozymes in pancreatic islets of the mouse [J].
Kim, SS ;
Jun, K ;
Jeong, MJ ;
Ryu, SH ;
Suh, PG ;
Shin, HS .
EXPERIMENTAL AND MOLECULAR MEDICINE, 2001, 33 (03) :164-168
12