Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry

被引：27

作者：

Belsom, Adam ^{[1
]}

Mudd, Gemma ^{[2
,3
]}

Giese, Sven ^{[4
]}

Auer, Manfred ^{[2
,3
]}

Rappsilber, Juri ^{[1
,4
]}

机构：

[1] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Inst Cell Biol, Sch Biol Sci, Edinburgh EH9 3BF, Midlothian, Scotland

[2] Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3BF, Midlothian, Scotland

[3] Univ Edinburgh, Med Sch, Edinburgh EH9 3BF, Midlothian, Scotland

[4] Tech Univ Berlin, Inst Biotechnol, Chair Bioanalyt, D-13355 Berlin, Germany

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 10期

基金：

英国惠康基金;

关键词：

HUMAN SERUM-ALBUMIN; ASSISTED-LASER-DESORPTION/IONIZATION; MASS-SPECTROMETRY; ACIDIC RESIDUES; PROTEINS; LINKERS;

D O I：

10.1021/acs.analchem.6b04938

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo cross-linking to a factor of 20x over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to different protein regions, unreachable with a single cross-linker.

引用

页码：5319 / 5324

页数：6

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