Application of a Mytilus edulis-derived promoting calcium absorption peptide in calcium phosphate cements for bone

被引：27
作者：
Xu, Zhe [1 ]
Zhu, Zhixuan [1 ]
Chen, Hui [3 ]
Han, Lingyu [1 ]
Shi, Pujie [2 ]
Dong, Xiufang [4 ]
Wu, Di [2 ]
Du, Ming [2 ]
Li, Tingting [1 ]
机构：
[1] Dalian Minzu Univ, Coll Life Sci, Key Lab Biotechnol & Bioresources Utilizat, Minist Educ, Dalian 116600, Peoples R China
[2] Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Collaborat Innovat Ctr Seafood Deep Proc, Sch Food Sci & Technol, Dalian 116034, Peoples R China
[3] Zhejiang Univ Technol, Coll Food Sci & Technol, Key Lab Marine Fishery Resources Exploitment & Uti, Hangzhou 310014, Peoples R China
[4] Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
来源：
BIOMATERIALS | 2022年 / 282卷
基金：
国家重点研发计划;
关键词：
Mytilus edulis; Peptide; Absorption; Calcium phosphate cements; Bone; THR-LYS-GLU; IN-VITRO; IRON-BINDING; BIOAVAILABILITY; DIFFERENTIATION; IDENTIFICATION; NANOCOMPOSITE; SOLUBILITY; CHELATION; COMPOSITE;
D O I：
10.1016/j.biomaterials.2022.121390
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The IEELEEELEAER peptide (PIE) identified from the protein hydrolysate of Mytilus edulis is reported to enhance osteoblast growth and differentiation, which also possesses a superior bone formation ability both in vitro and in vivo. Moreover, PIE bound to calcium spontaneously at the stoichiometry of 1:1, and there were amino nitrogen and carboxyl oxygen atoms in 2 glutamic acid residues at the calcium-binding sites in the PIE. The PIE-calcium complex facilitated calcium uptake through the Caco-2 cell monolayers. Incorporation of PIE into calcium phosphate cements enhanced calcium ion uptake and proliferation of osteoblasts and inhibit bacteria. This study suggest that calcium phosphate cements supplemented with PIE can serve as a potentially efficient material for bone graft used during spinal surgery.
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页数：9
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