Evaluation of the infection process by Lecanicillium fungicola in Agaricus bisporus by scanning electron microscopy

被引：10

作者：

Nunes, Janaira Santana ^{[1
]}

de Brito, Manuela Rocha ^{[1
]}

Zied, Diego Cunha ^{[2
]}

das Gracas Leite, Eloisa Aparecida ^{[1
]}

Dias, Eustaquio Souza ^{[1
]}

Alves, Eduardo ^{[1
]}

机构：

[1] Univ Fed Lavras, BR-37200000 Lavras, MG, Brazil

[2] Univ Estadual Paulista UNESP, Fac Ciancias Agrarias & Tecnolog, Campus Dracena, BR-17900000 Dracena, SP, Brazil

来源：

REVISTA IBEROAMERICANA DE MICOLOGIA | 2017年 / 34卷 / 01期

关键词：

Scanning electron microscopy; Mushroom; X-ray microanalyses; Mycopathogen; MYCOPARASITE VERTICILLIUM-FUNGICOLA; CULTIVATED MUSHROOMS; DRY BUBBLE; DISEASE; HOST; PATHOGENICITY; LECTIN; HYPHAE; SOIL;

D O I：

10.1016/j.riam.2016.04.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Lecanicillium fungicola causes dry bubble disease in Agaricus bisporus mushrooms leading to significant economic losses in commercial production. Aims: To monitor the infection process of L fungicola in Brazilian strains of A. bisporus. Methods: The interaction between the mycelium of L. fungicola (LF.1) and three strains of A. bisporus (ABI 7, ABI 11/14 and ABI 11/21) was studied. Electron microscopy and X-ray microanalyses of vegetative growth and basidiocarp infection were evaluated. Results: Micrographs show that the vegetative mycelium of the Brazilian strains of A. bisporus is not infected by the parasite. The images show that the pathogen caninterlace the hyphae of A. bisporus without causing damage, which contributes to the presence of L. fungicola during the substrate colonization, allowing their presence during primordial formation of A. bisporus. In the basidiocarp, germ tubes form within 16 h of infection with L. fungicola and the beginning of penetration takes place within 18 h, both without the formation of specialized structures. Conclusions: Scanning electron microscopy enabled the process of colonization and reproduction to be observed within the formation of phialides, conidiophores and verticils of L fungicola. The formation of calcium oxalate crystals by the pathogen was also visible using the X-ray microanalysis, both at the hyphae in the Petri plate and at basidiocarp infection site. (C) 2016 Asociacion Espaisiola de Micologia. Published by Elsevier Espafia, S.L.U. All rights reserved.

引用

页码：36 / 42

页数：7

共 26 条

[1]

Berendsen RL, 2010, MOL PLANT PATHOL, V11, P585, DOI [10.1111/J.1364-3703.2010.00627.X, 10.1111/j.1364-3703.2010.00627.x]

[2]

Bernardo D, 2004, CAN J MICROBIOL, V50, P729, DOI [10.1139/w04-047, 10.1139/W04-047]

[3]

Bozzola J.J., 1998, Electron Microscopy Principles and Techniques for Biologists, V3nd

[4] Interaction between the mycoparasite Verticillium fungicola and the vegetative mycelial phase of Agaricus bisporus [J].

Calonje, M ;

Mendoza, CG ;

Cabo, AP ;

Bernardo, D ;

Novaes-Ledieu, M .

MYCOLOGICAL RESEARCH, 2000, 104 :988-992

[5] Properties of a hydrophobin isolated from the mycoparasitic fungus Verticillium fungicola [J].

Calonje, M ;

Bernardo, D ;

Novaes-Ledieu, M ;

Mendoza, CG .

CANADIAN JOURNAL OF MICROBIOLOGY, 2002, 48 (11) :1030-1034

[6] Enzymic activity of the mycoparasite Verticillium fungicola on Agaricus bisporus fruit body cell walls [J].

Calonje, M ;

Mendoza, CG ;

Galan, B ;

NovaesLedieu, M .

MICROBIOLOGY-UK, 1997, 143 :2999-3006

[7] The pmk1-like mitogen-activated protein kinase from Lecanicillium (Verticillium) fungicola is not required for virulence on Agaricus bisporus [J].

Collopy, Patrick D. ;

Amey, Richard C. ;

Sergeant, Martin J. ;

Challen, Michael P. ;

Mills, Peter R. ;

Foster, Gary D. ;

Bailey, Andy M. .

MICROBIOLOGY-SGM, 2010, 156 :1439-1447

[8] Intracellular infection of the cultivated mushroom Agaricus bisporus by the mycoparasite Verticillium fungicola var fungicola [J].

Dragt, JW ;

Geels, FP ;

DeBruijn, WC ;

VanGriensven, LJLD .

MYCOLOGICAL RESEARCH, 1996, 100 :1082-1086

[9] Oxalate production by fungi: Its role in pathogenicity and ecology in the soil environment [J].

Dutton, MV ;

Evans, CS .

CANADIAN JOURNAL OF MICROBIOLOGY, 1996, 42 (09) :881-895

[10] Reduced sensitivity of the mushroom pathogen Verticillium fungicola to prochloraz-manganese in vitro [J].

Gea, FJ ;

Navarro, MJ ;

Tello, JC .

MYCOLOGICAL RESEARCH, 2005, 109 :741-745

← 1 2 3 →