Getting the right answers: understanding metabolomics challenges

被引:48
作者
Beisken, Stephan [1 ]
Eiden, Michael [2 ]
Salek, Reza M. [1 ,3 ,4 ]
机构
[1] European Bioinformat Inst, European Mol Biol Lab, Welcome Trust, Hinxton CB10 1SD, Cambs, England
[2] Metabolomx, Mountain View, CA 94043 USA
[3] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England
[4] Univ Cambridge, Cambridge Syst Biol Ctr, Cambridge CB2 1GA, England
关键词
community standards; data processing; design of experiments; mass spectrometry; metabolites; metabolome; metabolomics; nuclear magnetic resonance; small molecules; CHROMATOGRAPHY-MASS SPECTROMETRY; STANDARD REPORTING REQUIREMENTS; NUCLEAR-MAGNETIC-RESONANCE; LC-MS; BACKGROUND SUBTRACTION; GAS-CHROMATOGRAPHY; SPECTRA EXTRACTION; BIOLOGICAL SAMPLES; SMALL MOLECULES; PEAK DETECTION;
D O I
10.1586/14737159.2015.974562
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Small molecules within biological systems provide powerful insights into the biological roles, processes and states of organisms. Metabolomics is the study of the concentrations, structures and interactions of these thousands of small molecules, collectively known as the metabolome. Metabolomics is at the interface between chemistry, biology, statistics and computer science, requiring multidisciplinary skillsets. This presents unique challenges for researchers to fully utilize the information produced and to capture its potential diagnostic power. A good understanding of study design, sample preparation, analysis methods and data analysis is essential to get the right answers for the right questions. We outline the current state of the art, benefits and challenges of metabolomics to create an understanding of metabolomics studies from the experimental design to data analysis.
引用
收藏
页码:97 / 109
页数:13
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