Construction of constitutive expression of Eimeria tenella eukaryotic initiation factor U6L5H2 on the surface of Lactobacillus plantarum and evaluation of its immunoprotective efficiency against chicken coccidiosis

被引：3

作者：

Sun, Lingyu ^{[1
]}

Lu, Yaru ^{[1
]}

Zhao, Ningning ^{[1
]}

Wang, Yakun ^{[1
]}

Wang, Bingxiang ^{[1
]}

Li, Huihui ^{[1
]}

Wu, Zhiyuan ^{[1
]}

Li, Hongmei ^{[1
]}

Zhang, Xiao ^{[1
,4
]}

Zhao, Xiaomin ^{[1
,2
,3
,4
]}

机构：

[1] Shandong Agr Univ, Coll Vet Med, Dept Prevent Vet Med, Tai An, Shandong, Peoples R China

[2] Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contro, Tai An, Shandong, Peoples R China

[3] Shandong Agr Univ, Shandong Prov Engn Technol Res Ctr Anim Dis Contro, 61 Daizong St, Tai An 271018, Shandong, Peoples R China

[4] Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contro, 61 Daizong St, Tai An 271018, Shandong, Peoples R China

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2022年 / 252卷

关键词：

Constitutive expression; Eimeria tenella; Lactobacillus plantarum; Oral vaccine; Surface display; GENE-EXPRESSION; PROTECTIVE IMMUNITY; DNA VACCINE; PROTEIN; SUPPLEMENTATION; PROBIOTICS; STABILITY; INFECTION; PROMOTER; EFFICACY;

D O I：

10.1016/j.molbiopara.2022.111527

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lactobacillus strains exhibit preferable properties that make them attractive candidates for vaccine delivery systems because of their ability to regulate intestinal mucosal immunity in the body. To date, live Lactobacillus delivery vaccines reported for the defense against Eimeria tenella have been inducer-dependent systems whose applications are significantly limited due to their unattainable induction conditions in vivo. Here, a constitutive expression of Lactobacillus plantarum NC8 surface display system was constructed. Then, this system was used to prepare a live oral vaccine to constitutively express the E. tenella U6L5H2 (EtU6) protein on the NC8 surface and to evaluate its protective efficacy against E. tenella challenge in chickens. The results showed that the heterologous protein (EGFP or EtU6) was successfully expressed on the surface of L. plantarum NC8 without any inducer. The immunoprotection of EtU6 with constitutive expression in L. plantarum NC8 system (NC8/Pc-EtU6) was significantly stronger than that of EtU6 with induced expression of L. plantarum NC8 system (NC8/Pi-EtU6) (ACI: 168.28 vs. 152.74) as evidenced by increased body weight, decreased oocyst output and lesion scores. Furthermore, the constitutive system NC8/Pc-EtU6 produced higher levels of specific cecal SIgA, serum IgG, transcription of cytokines IFN-gamma and IL-2, and lymphocyte proliferation than the induced system NC8/Pi-EtU6. These results indicate that, compared to the inducible system, the constitutive surface display system of L. plantarum has the advantages of continuously expressing antigens in vivo and stimulating the host immune system. It could be an ideal platform for vaccine expression. The live vector vaccine for coccidiosis constructed by this constitutive system greatly improves the application potential in chicken production and provides a novel platform for the prevention of coccidiosis in chickens.

引用

页数：10